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The salicylic acid-induced protection of non-climacteric unripe pepper fruit against Colletotrichum gloeosporioides is similar to the resistance of ripe fruit

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Abstract

The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment protects unripe pepper fruit against the fungus, while jasmonic acid (JA) does not. The SA-mediated LAR in the unripe fruit inhibited the fungal appressoria, resulting in protection against fungal infection. Microarray analysis revealed that 177 of 7,900 cDNA clones showed more than fourfold transcriptional accumulation in SA-treated unripe fruit. The reverse transcription-polymerase chain reaction showed that most of the SA-responsive genes (SRGs) were regulated by SA, but not by JA or ethylene-releasing ethephon. Furthermore, most of the SRGs were preferentially expressed in the ripe fruit. These results suggest that the SA-mediated transcriptional regulation of SRGs has a critical role in the resistance of ripe pepper fruit to fungal infection.

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Abbreviations

LAR:

Local acquired resistance

SA:

Salicylic acid

INA:

2,6-Dichloroisonicotinic acid

BTH:

Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester

SRGs:

SA-responsive genes

JA:

Jasmonic acid

ET:

Ethylene

AR:

Acquired resistance

SAR:

Systemic acquired resistance

ISR:

Induced systemic resistance

RT-PCR:

Reverse transcription-polymerase chain reaction

DEPC:

Diethylpyrocarbonate

DAI:

Day after inoculation

PR:

Pathogenesis related

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Acknowledgments

This research was supported by a grant (CG1134) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government and a grant (Code 20070401-034-026) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Bio-application Team, Dongbu Advanced Research Institute, 103-2 Munji-dong, Daeduck Science Town, Daejeon, 305-708, Korea

    Sanghyeob Lee & Soonkee Sung

  2. Department of Biochemistry, Gyeongsang National University, 900 Gajwa-dong, Jinju, 660-701, Korea

    Jong-Chan Hong

  3. Plant and Microbe Co., Ltd in Biotechnology Industrialization Center, 880-4 Ansan-ri, Noan-myun, Naju, 520-811, Korea

    Woong Bae Jeon

  4. Department of Plant Genetic Engineering, Dong-A University, 840 Hadan 2-dong, Saha-gu, Busan, 604-714, Korea

    Young-Soo Chung

  5. Horticultural Science Program, Seoul National University, 599 Kwanakro, Kwanak-gu, Seoul, 151-742, Korea

    Doil Choi

  6. School of Biological Sciences and Technology, Jeonnam National University, 300 Youngbong-dong, Boog-gu, Kwangju, 500-757, Korea

    Young Hee Joung

  7. BioControlCenter, 272 ChangJung-ri, Ip-myon, Goksung-gun, Jeollanam-do, 516-942, Korea

    Boung-Jun Oh

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  1. Sanghyeob Lee
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  7. Young Hee Joung
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Correspondence to Young Hee Joung or Boung-Jun Oh.

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Communicated by J. R. Liu.

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Lee, S., Hong, JC., Jeon, W.B. et al. The salicylic acid-induced protection of non-climacteric unripe pepper fruit against Colletotrichum gloeosporioides is similar to the resistance of ripe fruit. Plant Cell Rep 28, 1573–1580 (2009). https://doi.org/10.1007/s00299-009-0756-5

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  • DOI: https://doi.org/10.1007/s00299-009-0756-5

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