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Detection of QTL affecting fatty acid composition in the pig

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Abstract

We present a QTL genome scan for fatty acid composition in pigs. An F2 cross between Iberian × Landrace pigs and a regression approach fitting the carcass weight as a covariate for QTL identification was used. Chromosomes (Chrs) 4, 6, 8, 10, and 12 showed highly significant effects. The Chr 4 QTL influenced the linoleic content and both the fatty acid double-bond index and peroxidability index. In Chr 6 we found significant associations with the double-bond index and the unsaturated index of fatty acids. Chr 8 showed clear effects on the percentages of palmitic and palmitoleic fatty acids as well as the average chain length of fatty acids. In Chr 10 we detected a significant QTL for the percentage of myristic fatty acid, with an F value that was slightly above the genomewide threshold. The percentage of linolenic fatty acid was affected by a region on Chr 12. A nearly significant QTL for the content of gadoleic fatty acid was also detected in Chr 12. We also analyzed the genomic QTL distribution by a regression model that fits the backfat thickness as a covariate. Some of the QTL that were detected in our analysis could not be detected when the data were corrected by backfat thickness. This work shows how critical the selection of a covariate can be in the interpretation of results. This is the first report of a genome scan detection of QTL directly affecting fatty acid composition in pigs.

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Acknowledgements

We are grateful to Marcel Amills for reviewing the manuscript. We are indebted to Pere Borràs and Eva Ramells and to the staff of Nova Genètica S.A. and Copaga. Thanks to Miguel Angel Toro and Josep Gasa for useful discussions throughout the project. Betlem Cabrera, Meritxell Arqué, A. Quintana, and C. Barboni provided excellent technical assistance. Microsatellites were kindly provided by Max Rothschild (U.S. Pig Genome Coordinator, NRSP-8). We are grateful to P. Green, C.S. Haley, and D. Milan for their analysis and database programs. The Guadyerbas boars were a gift from the SIA “El dehesón del Encinar” (Toledo, Spain). The Landrace F0, F1, and F2 Iberian × Landrace pigs were bred at the Nova Genètica S.A. facilities. Alex Clop received a predoctoral fellowship from the Universitat Autònoma de Barcelona. This project was funded by the MCYT (AGF96-2510-C05 and AGF99-0284-CO2-02).

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Authors and Affiliations

  1. Department de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain

    Alex Clop, Albert Cercos, Anna Tomas, Agustina Coll, Josep M. Folch & Armand Sanchez

  2. Area de Genética y Mejora Animal, SGIT-INIA, Ctra. De la Coruña km. 7, Madrid, 28040, Spain

    Cristina Ovilo, Ana Fernandez, Carmen Barragan & Luis Silio

  3. Area de Producció Animal, Centre UdL-IRTA, C/ Alcalde Rovira Roure 177, Lleida, 25198, Spain

    Miguel Perez-Enciso, Luis Varona & Jose L. Noguera

  4. IRTA-CTC, Monells, 17121, Girona, Spain

    Isabel Diaz & Maria A. Oliver

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  1. Alex Clop
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  2. Cristina Ovilo
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Correspondence to Alex Clop.

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Clop, A., Ovilo, C., Perez-Enciso, M. et al. Detection of QTL affecting fatty acid composition in the pig . Mamm Genome 14, 650–656 (2003). https://doi.org/10.1007/s00335-002-2210-7

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