Skip to main content

Advertisement

SpringerLink
Log in

EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human

  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

A molecular understanding of porcine reproduction is of biological interest and economic importance. Our Midwest Consortium has produced cDNA libraries containing the majority of genes expressed in major female reproductive tissues, and we have deposited into public databases 21,499 expressed sequence tag (EST) gene sequences from the 3′ end of clones from these libraries. These sequences represent 10,574 different genes, based on sequence comparison among these data, and comparison with existing porcine ESTs and genes indicate as many as 4652 of these EST clusters are novel. In silico analysis identified sequences that are expressed in specific pig tissues or organs and confirmed the broad expression in pig for many genes ubiquitously expressed in human tissues. Furthermore, we have developed computer software to identify sequence similarity of these pig genes with their human counterparts, and to extract the mapping information of these human homologues from genome databases. We demonstrate the utility of this software for comparative mapping by localizing 61 genes on the porcine physical map for Chromosomes (Chrs) 5, 10, and 14.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. MD Adams JM Kelley JD Gocayne M Dubnick MH Polymeropoulos et al. (1991) ArticleTitleComplementary DNA sequencing: expressed sequence tags and human genome project. Science 252 1651–1656

    Google Scholar 

  2. SF Altschul W Gish W Miller EW Myers DJ Lipman (1990) ArticleTitleBasic local alignment search tool. J Mol Biol 215 403–410 Occurrence Handle10.1006/jmbi.1990.9999 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D Occurrence Handle2231712

    Article  CAS  PubMed  Google Scholar 

  3. S Audic JM Claverie (1997) ArticleTitleThe significance of digital gene expression profiles. Genome Res 7 986–995 Occurrence Handle1:CAS:528:DyaK2sXntFahurc%3D Occurrence Handle9331369

    CAS  PubMed  Google Scholar 

  4. MS Bektas FA Bieber G Braunitzer (1981) ArticleTitleHemoglobins, XLI. The embryonic hemoglobins of mammal. A new hemoglobin, hemoglobin Heide II (Hb HeII: alpha 2 epsilon 2), and demonstration of the hemoglobin structure of gower I (zeta 3 epsilon 2) in the pig embryo. Hoppe Seyler's Z Physiol Chem 362 991–995 Occurrence Handle1:CAS:528:DyaL3MXlt1OksbY%3D Occurrence Handle6168553

    CAS  PubMed  Google Scholar 

  5. MF Bonaldo G Lennon MB Scares (1996) ArticleTitleNormalization and subtraction: two approaches to facilitate gene discovery. Genome Res 6 791–806 Occurrence Handle1:CAS:528:DyaK28XlvF2ns7Y%3D Occurrence Handle8889548

    CAS  PubMed  Google Scholar 

  6. Caetano AR, Johnson RK, Pomp D (2002) RNA expression profiling of ovarian follicle development on swine lines selected for increased ovulation rate. In 7th World Congress on Genetics Applied to Livestock Production. CD-ROM communication N° 08-07 (ISBN 2-7380-1052-0)

  7. AR Caetano RK Johnson D Pomp (2003) ArticleTitleGeneration and sequences characterization of a normalized cDNA library from swine ovarian follicles. Mamm Genome 14 65–70 Occurrence Handle10.1007/s00335-002-2220-5 Occurrence Handle1:CAS:528:DC%2BD3sXltlCjug%3D%3D Occurrence Handle12532269

    Article  CAS  PubMed  Google Scholar 

  8. P Carpintero F Franco del Amo R Anadon J Gomez-Marquez (1996) ArticleTitleThymosin beta 10 mRNA expression during early postimplantation mouse development. FEES Lett 394 103–106 Occurrence Handle10.1016/0014-5793(96)00888-5 Occurrence Handle1:CAS:528:DyaK28XlvVagurg%3D

    Article  CAS  Google Scholar 

  9. JP Cassady RK Johnson D Pomp GA Rohrer LD Van Vleck et al. (2001) ArticleTitleIdentification of quantitative trait loci affecting reproduction in pigs. J Anim Sci 79 623–633 Occurrence Handle1:CAS:528:DC%2BD3MXjsVygtLo%3D Occurrence Handle11263822

    CAS  PubMed  Google Scholar 

  10. A Cencic C La Bonnardiere (2002) ArticleTitleTrophoblastic interferon-gamma: current knowledge and possible role(s) in early pig pregnancy. Vet Res 33 139–157 Occurrence Handle10.1051/vetres:2002003 Occurrence Handle1:CAS:528:DC%2BD38XjsVyqu70%3D Occurrence Handle11944804

    Article  CAS  PubMed  Google Scholar 

  11. I Choi RC Simmen FA Simmen (1996) ArticleTitleMolecular cloning of cytochrome P450 aromatase complementary deoxyribonucleic acid from periimplantation porcine and equine blastocysts identifies multiple novel 5′-untranslated exons expressed in embryos, endometrium, and placenta. Endocrinology 137 1457–1467 Occurrence Handle1:CAS:528:DyaK28XhvVegtLY%3D Occurrence Handle8625924

    CAS  PubMed  Google Scholar 

  12. BP Chowdhary T Raudsepp L Fronicke H Scherthan (1998) ArticleTitleEmerging patterns of comparative genome organization in some mammalian species as revealed by Zoo-FISH. Genome Res 8 577–589 Occurrence Handle1:CAS:528:DyaK1cXkt1aruro%3D Occurrence Handle9647633

    CAS  PubMed  Google Scholar 

  13. C Clouscard-Martinato P Mulsant A Robic A Bonnet F Gasser et al. (1998) ArticleTitleCharacterization of FSH-regulated genes isolated by mRNA differential display from pig ovarian granulosa cells. Anim Genet 29 98–106 Occurrence Handle10.1046/j.1365-2052.1998.00279.x Occurrence Handle1:CAS:528:DyaK1cXlt1arsr4%3D Occurrence Handle9699269

    Article  CAS  PubMed  Google Scholar 

  14. R Davoli L Fontanesi P Zambonelli D Bigi J Gellin et al. (2002) ArticleTitleIsolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig. Anim Genet 33 3–18 Occurrence Handle10.1046/j.1365-2052.2002.00800.x Occurrence Handle1:CAS:528:DC%2BD38XhsFShsb0%3D Occurrence Handle11849132

    Article  CAS  PubMed  Google Scholar 

  15. P Deloukas GD Schuler G Gyapay EM Beasley C Soderlund et al. (1998) ArticleTitleA physical map of 30,000 human genes. Science 282 744–746 Occurrence Handle1:CAS:528:DyaK1cXmvFOqu74%3D Occurrence Handle9784132

    CAS  PubMed  Google Scholar 

  16. B Ewing P Green (1998) ArticleTitleBase-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res 8 186–194 Occurrence Handle1:CAS:528:DyaK1cXitlWlu7g%3D Occurrence Handle9521922

    CAS  PubMed  Google Scholar 

  17. SC Fahrenkrug TP Smith BA Freking J Cho J White et al. (2002) ArticleTitlePorcine gene discovery by normalized cDNA-library sequencing and EST cluster assembly. Mamm Genome 13 475–478 Occurrence Handle12226715

    PubMed  Google Scholar 

  18. J Flint R Mott (2001) ArticleTitleFinding the molecular basis of quantitative traits: successes and pitfalls. Nat Rev Genet 2 437–445 Occurrence Handle10.1038/35076585 Occurrence Handle1:CAS:528:DC%2BD3MXktlKqurY%3D Occurrence Handle11389460

    Article  CAS  PubMed  Google Scholar 

  19. AJ Gavin TE Scheetz CA Roberts B O'Leary TA Braun et al. (2002) ArticleTitlePooled library tissue tags for EST-based gene discovery. Bioinformatics 18 1162–1166 Occurrence Handle10.1093/bioinformatics/18.9.1162 Occurrence Handle1:CAS:528:DC%2BD38XmvVCqsLg%3D Occurrence Handle12217907

    Article  CAS  PubMed  Google Scholar 

  20. A Goureau M Yerle A Schmitz J Riquet D Milan et al. (1996) ArticleTitleHuman and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36 252–262 Occurrence Handle10.1006/geno.1996.0460 Occurrence Handle1:CAS:528:DyaK28XlslWiurs%3D Occurrence Handle8812451

    Article  CAS  PubMed  Google Scholar 

  21. RJ Hawken J Murtaugh GH Flickinger M Yerle A Robic et al. (1999) ArticleTitleA first-generation porcine whole-genome radiation hybrid map. Mamm Genome 10 824–830 Occurrence Handle1:CAS:528:DyaK1MXkvV2msLY%3D Occurrence Handle10430669

    CAS  PubMed  Google Scholar 

  22. H Hirooka DJ de Koning B Harlizius JA van Arendonk AP Rattink et al. (2001) ArticleTitleA whole-genome scan for quantitative trait loci affecting teat number in pigs. J Anim Sci 79 2320–2326 Occurrence Handle1:CAS:528:DC%2BD3MXntFWisbc%3D Occurrence Handle11583418

    CAS  PubMed  Google Scholar 

  23. LL Hsiao F Dangond T Yoshida R Hong RV Jensen et al. (2001) ArticleTitleA compendium of gene expression in normal human tissues. Physiol Genomics 7 97–104 Occurrence Handle1:CAS:528:DC%2BD38XhtFagsLs%3D Occurrence Handle11773596

    CAS  PubMed  Google Scholar 

  24. InstitutionalAuthorNameInternational Human Genome Sequencing Consortium (2001) ArticleTitleInitial sequencing and analysis of the human genome. Nature 409 860–921 Occurrence Handle1:CAS:528:DC%2BD3MXhsFCjtLc%3D Occurrence Handle11237011

    CAS  PubMed  Google Scholar 

  25. HJ Jacob AE Kwitek (2002) ArticleTitleRat genetics: attaching physiology and pharmacology to the genome. Nat Rev Genet 3 33–42 Occurrence Handle10.1038/nrg702 Occurrence Handle1:CAS:528:DC%2BD38XhsV2gsb0%3D Occurrence Handle11823789

    Article  CAS  PubMed  Google Scholar 

  26. MA Kaminski SP Ford AJ Conley (1997) ArticleTitleDifferences in the expression of cytochromes P450 17 alpha-hydroxylase and aromatase in Meishan and Yorkshire conceptuses at days 10.5–14.0 of gestation. J Reprod Fertil 111 213–219 Occurrence Handle1:CAS:528:DyaK1cXlsFeksg%3D%3D Occurrence Handle9462288

    CAS  PubMed  Google Scholar 

  27. L Lai D Kolber-Simonds KW Park HT Cheong JL Greenstein et al. (2002) ArticleTitleProduction of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295 1089–1092 Occurrence Handle10.1126/science.1068228 Occurrence Handle1:CAS:528:DC%2BD38Xht1Gqtbw%3D Occurrence Handle11778012

    Article  CAS  PubMed  Google Scholar 

  28. NJ Larsen S Marklund KA Kelly M Malek CK Tuggle et al. (1999) ArticleTitleNew insights into porcine-human synteny conservation. Mamm Genome 10 488–491 Occurrence Handle10.1007/s003359901028 Occurrence Handle1:CAS:528:DyaK1MXkt1Oltro%3D Occurrence Handle10337623

    Article  CAS  PubMed  Google Scholar 

  29. RC Levitt (1991) ArticleTitlePolymorphisms in the transcribed 3′ untranslated region of eukaryotic genes. Genomics 11 484–489 Occurrence Handle1:CAS:528:DyaK38XhsVCrtrY%3D Occurrence Handle1769669

    CAS  PubMed  Google Scholar 

  30. MD Li RL Matteri GJ Macdonald TH Wise JJ Ford (1996) ArticleTitleOverexpression of beta-subunit of thyroid-stimulating hormone in Meishan swine identified by differential display. J Anim Sci 74 2104–2111 Occurrence Handle1:CAS:528:DyaK28Xls1Ogu7s%3D Occurrence Handle8880412

    CAS  PubMed  Google Scholar 

  31. MA Marra TA Kucaba NL Dietrich ED Green B Brownstein et al. (1997) ArticleTitleHigh throughput fingerprint analysis of large-insert clones. Genome Res 7 1072–1084 Occurrence Handle1:CAS:528:DyaK2sXns1Oisrc%3D Occurrence Handle9371743

    CAS  PubMed  Google Scholar 

  32. LA Messer L Wang CK Tuggle M Yerle P Chardon et al. (1997) ArticleTitleMapping of the melatonin receptor 1a (MTNR1A) gene in pigs, sheep, and cattle. Mamm Genome 8 368–370 Occurrence Handle10.1007/s003359900444 Occurrence Handle1:CAS:528:DyaK2sXjtVOhsbc%3D Occurrence Handle9107687

    Article  CAS  PubMed  Google Scholar 

  33. Y Okazaki M Furuno T Kasukawa J Adachi H Bono et al. (2002) ArticleTitleAnalysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature 420 563–573 Occurrence Handle10.1038/nature01266 Occurrence Handle12466851

    Article  PubMed  Google Scholar 

  34. BM Patten (1948) Embryology of the Pig. McGraw-Hill Book Company New York

    Google Scholar 

  35. JS Perry (1954) ArticleTitleFecundity and embryonic mortality in pigs. J Embryol Exp Morphol 2 308–322

    Google Scholar 

  36. A Rink EM Santschi CW Beattie (2002a) ArticleTitleNormalized cDNA libraries from a porcine model of orthopedic implant-associated infection. Mamm Genome 13 198–205 Occurrence Handle1:CAS:528:DC%2BD38XjtFymt70%3D

    CAS  Google Scholar 

  37. A Rink EM Santschi KM Eyer B Roelofs M Hess et al. (2002b) ArticleTitleA first-generation EST RH comparative map of the porcine and human genome. Mamm Genome 13 578–587 Occurrence Handle1:CAS:528:DC%2BD38Xos1amu7c%3D

    CAS  Google Scholar 

  38. GA Rohrer LJ Alexander Z Hu TP Smith JW Keele et al. (1996) ArticleTitleA comprehensive map of the porcine genome. Genome Res 6 371–391 Occurrence Handle1:CAS:528:DyaK28Xjtlymsb4%3D Occurrence Handle8743988

    CAS  PubMed  Google Scholar 

  39. GA Rohrer JJ Ford TH Wise JL Vallet RK Christenson (1999) ArticleTitleIdentification of quantitative trait loci affecting female reproductive traits in a multigeneration Meishan-White composite swine population. J Anim Sci 77 1385–1391 Occurrence Handle1:CAS:528:DyaK1MXjs1eqtrc%3D Occurrence Handle10375216

    CAS  PubMed  Google Scholar 

  40. JW Ross MD Ashworth AG Hurst JR Malayer RD Geisert (2002) ArticleTitleDifferential gene expression during trophoblast elongation of porcine conceptuses. Biol Reprod Suppl 1 105

    Google Scholar 

  41. Scheetz TE, Trivedi N, Roberts, CA, Kucaba T, Berger B, et al. ESTprep: preprocessing cDNA sequence reads. Bioinformatics, to appear

  42. M Schena D Shalon RW Davis PO Brown (1995) ArticleTitleQuantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270 467–470 Occurrence Handle1:CAS:528:DyaK2MXovVersLk%3D Occurrence Handle7569999

    CAS  PubMed  Google Scholar 

  43. GD Schuler MS Boguski EA Stewart LD Stein G Gyapay et al. (1996) ArticleTitleA gene map of the human genome. Science 274 540–546

    Google Scholar 

  44. Smit AFA, Green P (2001) Repeat Masker. http://repeatmasker.qenome.washington.edu/cqi-bin/Repeat Masker

  45. TP Smith SC Fahrenkrug GA Rohrer FA Simmen CE Rexroad et al. (2001) ArticleTitleMapping of expressed sequence tags from a porcine early embryonic cDNA library. Anim Genet 32 66–72 Occurrence Handle10.1046/j.1365-2052.2001.00739.x Occurrence Handle1:CAS:528:DC%2BD3MXltFajs7s%3D Occurrence Handle11421940

    Article  CAS  PubMed  Google Scholar 

  46. DJ Stekel Y Git F Falciani (2000) ArticleTitleThe comparison of gene expression from multiple cDNA libraries. Genome Res 10 2055–2061 Occurrence Handle10.1101/gr.GR-1325RR Occurrence Handle1:CAS:528:DC%2BD3MXjslY%3D Occurrence Handle11116099

    Article  CAS  PubMed  Google Scholar 

  47. RT Stone (1989) ArticleTitleIsolation and characterization of recombinant cDNA clones corresponding to developmentally regulated genes in pig liver. J Anim Sci 67 1082–1089 Occurrence Handle1:CAS:528:DyaL1MXktVWru74%3D Occurrence Handle2715113

    CAS  PubMed  Google Scholar 

  48. HS Sun CW Ernst M Yerle P Pinton MF Rothschild et al. (1999a) ArticleTitleComparative mapping and analysis of human chromosome 3 genes in the pig shows complete synteny but extensive arranged gene order. Cytogenet Cell Genet 85 273–278 Occurrence Handle1:CAS:528:DyaK1MXlvFShs7w%3D

    CAS  Google Scholar 

  49. HS Sun M Yerle P Pinton P Chardon et al. (1999b) ArticleTitlePhysical assignments of human chromosome 13 genes on pig chromosome 11 demonstrate extensive synteny and gene order conservation between pig and human. Anim Genet 30 304–308 Occurrence Handle1:CAS:528:DyaK1MXlvVagurg%3D

    CAS  Google Scholar 

  50. B Szafranska S Xie J Green RM Roberts (1995) ArticleTitlePorcine pregnancy-associated glycoproteins: new members of the aspartic proteinase gene family expressed in trophectoderm. Biol Reprod 53 21–28 Occurrence Handle1:CAS:528:DyaK2MXmt12it70%3D Occurrence Handle7669851

    CAS  PubMed  Google Scholar 

  51. DW Threadgill KW Hunter RW Williams (2002) ArticleTitleGenetic dissection of complex and quantitative traits: from fantasy to reality via a community effort. Mamm Genome 13 175–178 Occurrence Handle10.1007/s00335-001-4001-Y Occurrence Handle1:CAS:528:DC%2BD38XjtFymtrY%3D Occurrence Handle11956758

    Article  CAS  PubMed  Google Scholar 

  52. N Trivedi J Bischof S Davis KT Pedretti et al. (2002) ArticleTitleParallel creation of non-redundant gene indices from partial mRNA transcripts. Future Generation Comput Syst 18 863–870 Occurrence Handle10.1016/S0167-739X(02)00059-6

    Article  Google Scholar 

  53. CK Tuggle CB Schmitz (1994) ArticleTitleCloning and characterization of pig muscle cDNA by an expressed sequence tag approach. Anim Biotechnol 5 1–13 Occurrence Handle1:CAS:528:DyaK2MXmt1OgtQ%3D%3D

    CAS  Google Scholar 

  54. CK Tuggle C Genet XW Shi P Chardon I Sanchez-Serrano et al. (2001) ArticleTitleCytogenetic and radiation hybrid mapping reveals conserved synteny and gene order between human chromosome 21 and pig chromosome 13. Mamm Genome 12 397–399 Occurrence Handle10.1007/s003350020004 Occurrence Handle1:CAS:528:DC%2BD3MXjt1Chsr4%3D Occurrence Handle11331950

    Article  CAS  PubMed  Google Scholar 

  55. W Tuo JP Harney FW Bazer (1996) ArticleTitleDevelopmentally regulated expression of interleukin-1 beta by peri-implantation conceptuses in swine. J Reprod Immunol 31 185–198 Occurrence Handle10.1016/0165-0378(96)00975-8 Occurrence Handle1:CAS:528:DyaK28XntFCltLk%3D Occurrence Handle8905551

    Article  CAS  PubMed  Google Scholar 

  56. J Ullman (1982) Principles of Database System. Computer Science Press Rockville, Md.

    Google Scholar 

  57. WJ Van Etten RG Steen H Nguyen AB Castle DK Slonim et al. (1999) ArticleTitleRadiation hybrid map of the mouse genome. Nat Genet 22 384–387 Occurrence Handle10.1038/11962 Occurrence Handle1:CAS:528:DyaK1MXltVSgtrc%3D Occurrence Handle10431245

    Article  CAS  PubMed  Google Scholar 

  58. RH Waterston K Lindblad-Toh E Birney J Rogers JF Abril et al. (2002) ArticleTitleInitial sequencing and comparative analysis of the mouse genome. Nature 420 520–562 Occurrence Handle10.1038/nature01262 Occurrence Handle12466850

    Article  PubMed  Google Scholar 

  59. KM Whitworth MC Lucy JE Ries GK Springer A Guillen et al. (2002) ArticleTitleCharacterization of cDNA libraries developed from porcine oocytes and embryos: identification of differentially expressed genes. Biol Reprod Suppl 1 159

    Google Scholar 

  60. PJ Wilkie AA Paszek CW Beattie LJ Alexander MB Wheeler et al. (1999) ArticleTitleA genomic scan of porcine reproductive traits reveals possible quantitative trait loci (QTLs) for number of corpora lutea. Mamm Genome 10 573–578 Occurrence Handle10.1007/s003359901047 Occurrence Handle1:CAS:528:DyaK1MXkt1OltLw%3D Occurrence Handle10341088

    Article  CAS  PubMed  Google Scholar 

  61. ME Wilson TS Sonstegard TP Smith SC Fahrenkrug SP Ford (2000) ArticleTitleDifferential gene expression during elongation in the preimplantation pig embryo. Genesis 26 9–14 Occurrence Handle10.1002/(SICI)1526-968X(200001)26:1<9::AID-GENE4>3.0.CO;2-1 Occurrence Handle1:CAS:528:DC%2BD3cXhtlCgt7s%3D Occurrence Handle10660669

    Article  CAS  PubMed  Google Scholar 

  62. ME Wilson SC Fahrenkrug TP Smith GA Rohrer SP Ford (2002) ArticleTitleDifferential expression of cyclooxygenase-2 around the time of elongation in the pig conceptus. Anim Reprod Sci 71 229–237 Occurrence Handle10.1016/S0378-4320(02)00029-5 Occurrence Handle1:CAS:528:DC%2BD38XktFyiur8%3D Occurrence Handle12047931

    Article  CAS  PubMed  Google Scholar 

  63. AK Wintero M Fredholm L Andersson (1994) ArticleTitleAssignment of the gene for porcine insulin growth factor 1 (IGF1) to chromosome 5 by linkage mapping. Anim Genet 25 37–39 Occurrence Handle1:CAS:528:DyaK2MXpsF2r

    CAS  Google Scholar 

  64. AK Winterø M Fredholm W Davies (1996) ArticleTitleEvaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones. Mamm Genome 7 509–517 Occurrence Handle10.1007/s003359900153 Occurrence Handle8672129

    Article  PubMed  Google Scholar 

  65. JV Yelich D Pomp RD Geisert (1997) ArticleTitleOntogeny of elongation and gene expression in the early developing porcine conceptus. Biol Reprod 57 1256–1265 Occurrence Handle1:CAS:528:DyaK2sXmvFSkur4%3D Occurrence Handle9369195

    CAS  PubMed  Google Scholar 

  66. M Yerle G Echard A Robic A Mairal C Dubut-Fontana et al. (1996) ArticleTitleA somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet Cell Genet 73 194–202 Occurrence Handle1:CAS:528:DyaK28XkvVGjs74%3D Occurrence Handle8697807

    CAS  PubMed  Google Scholar 

  67. M Yerle P Pinton A Robic A Alfonso Y Palvadeau et al. (1998) ArticleTitleConstruction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet 82 182–188 Occurrence Handle10.1159/000015095 Occurrence Handle1:CAS:528:DyaK1MXkslyqtQ%3D%3D Occurrence Handle9858812

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by USDA-NRICGP 99-35205-8370 and by the office of the Vice-Provost, Iowa State University. This journal paper of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project No. 3596, was also supported by the Hatch Act and State of Iowa funds. We gratefully acknowledge the contributions of Darla Hartzler, Katie Barry, Catherine Keppel, Kurtis Trout, Mark Lebeck, Katrina Fishler, and Kelly Schaefer for technical assistance; Chad Roberts for bioinformatic support; and Sara Olberding and Kerry Burns in generating mapping data. Also published as paper number 13955 of the journal series, Nebraska Agricultural Experiment Station.

Author information

Authors and Affiliations

  1. Center for Integrated Animal Genomics and Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, Iowa 50011, USA

    Christopher K. Tuggle, Carolyn Fitzsimmons, Yuandan Zhang & Max F. Rothschild

  2. Department of Animal Science, University of Missouri-Columbia, Columbia, Missouri, USA

    Jon A. Green, Rami Woods & Randall S. Prather

  3. Departments of Pediatrics, Biochemistry, Orthopedics, Physiology and Biophysics, University of Iowa, Iowa City, Iowa, USA

    Sergei Malchenko, Bento M. Soares, Tamara Kucaba, Keith Crouch & Christina Smith

  4. Center for Bioinformatics and Computational Biology and Department of Biomedical Engineering, University of Iowa, Iowa City, Iowa, USA

    Dylan Tack, Natalie Robinson, Brian O'Leary & Thomas Casavant

  5. Center for Bioinformatics and Computational Biology and Department of Ophthalmology, University of Iowa, Iowa City, Iowa, USA

    Todd Scheetz

  6. Department of Animal Science, University of Nebraska, Lincoln, Nebraska, USA

    Daniel Pomp & Brad J. Edeal

  7. National Center for Genomic Resources, Sante Fe, New Mexico, USA

    Kevin Garwood & William Beavis

Authors
  1. Christopher K. Tuggle
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jon A. Green
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carolyn Fitzsimmons
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rami Woods
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Randall S. Prather
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sergei Malchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Bento M. Soares
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Tamara Kucaba
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Keith Crouch
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Christina Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Dylan Tack
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Natalie Robinson
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Brian O'Leary
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Todd Scheetz
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Thomas Casavant
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Daniel Pomp
    View author publications

    You can also search for this author in PubMed Google Scholar

  17. Brad J. Edeal
    View author publications

    You can also search for this author in PubMed Google Scholar

  18. Yuandan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  19. Max F. Rothschild
    View author publications

    You can also search for this author in PubMed Google Scholar

  20. Kevin Garwood
    View author publications

    You can also search for this author in PubMed Google Scholar

  21. William Beavis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Christopher K. Tuggle.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tuggle, C.K., Green, J.A., Fitzsimmons, C. et al. EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human . Mamm Genome 14, 565–579 (2003). https://doi.org/10.1007/s00335-002-2263-7

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00335-002-2263-7

Keywords

Search

Navigation