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Association between equine temperament and polymorphisms in dopamine D4 receptor gene

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Abstract

The variable number of tandem repeats (VNTR) polymorphism of the dopamine D4 receptor (DRD4) gene has been reported to be associated with the personality trait of novelty-seeking in humans. In the genus Equus, this region includes an 18-bp repeat unit and there are inter- and intraspecies differences in the number of repetitions. Because horses are unique among livestock species in that their temperament is considered important, we investigated the possible role of this region on equine temperament in thoroughbred horses. We simultaneously determined the sequences of this polymorphic region and administered a questionnaire survey to horse caretakers with questions about 20 different traits of their horses’ temperament. Although there was no difference in the number of repeats among the 136 thoroughbred horses studied, two single nucleotide polymorphisms (SNPs), one of which might cause an amino acid change (A-G substitution), existed. By analyzing the association between these SNPs and temperament scores, a significant association was revealed between two temperament traits (Curiosity and Vigilance) and the A-G substitution. Horses without the A allele had significantly higher Curiosity and lower Vigilance scores than those with the A allele at the A-G substitution. In addition, similar associations between both temperament scores and each genotype of the A-G substitution were observed in two subgroups divided according to the time of their introduction to the farm. These results suggested that the SNP in the VNTR region of the equine DRD4 gene might be related to individual differences in equine temperament.

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References

  • Benjamin J, Li L, Patterson C, Greenberg BD, Murphy DL, et al. (1996) Population and familial association between the D4 dopamine receptor gene and measures of Novelty Seeking. Nat Genet 12: 81-84

    Article  PubMed  Google Scholar 

  • Bland JM, Altman DG (1995) Multiple significance tests: the Bonferroni method. BMJ 310: 170

    PubMed  Google Scholar 

  • Catalano M, Nobile M, Novelli E, Nothen MM, Smeraldi E (1993) Distribution of a novel mutation in the first exon of the human dopamine D4 receptor gene in psychotic patients. Biol Psychiatry 34: 459-464

    Article  PubMed  Google Scholar 

  • Cichon S, Nothen MM, Catalano M, Di Bella D, Maier W, et al. (1995) Identification of two novel polymorphisms and a rare deletion variant in the human dopamine D4 receptor gene. Psychiatr Genet 5: 97-103

    PubMed  Google Scholar 

  • Cloninger CR, Svrakic DM, Przybeck TR (1993) A psychobiological model of temperament and character. Arch Gen Psychiatry 50: 975-990

    PubMed  Google Scholar 

  • Comings DE, MacMurray JP (2000) Molecular heterosis: a review. Mol Genet Metab 71: 19-31

    Article  PubMed  Google Scholar 

  • Ding YC, Chi HC, Grady DL, Morishima A, Kidd JR, et al. (2002) Evidence of positive selection acting at the human dopamine receptor D4 gene locus. Proc Natl Acad Sci U S A 99: 309-314

    Article  PubMed  Google Scholar 

  • Dulawa SC, Grandy DK, Low MJ, Paulus MP, Geyer MA (1999) Dopamine D4 receptor-knock-out mice exhibit reduced exploration of novel stimuli. J Neurosci 19: 9550-9556

    PubMed  Google Scholar 

  • Ebstein RP, Novick O, Umansky R, Priel B, Osher Y, et al. (1996) Dopamine D4 receptor (D4DR) exon III polymorphism associated with the human personality trait of Novelty Seeking. Nat Genet 12: 78-80

    Article  PubMed  Google Scholar 

  • Fishburn CS, Carmon S, Fuchs S (1995) Molecular cloning and characterisation of the gene encoding the murine D4 dopamine receptor. FEBS Lett 361: 215-219

    Article  PubMed  Google Scholar 

  • Hasegawa T, Sato F, Ishida N (2002) Determination and variability of nucleotide sequences for D4 dopamine receptor genes (DRD4) in genus Equus. J Equine Sci 13: 57-62

    Article  Google Scholar 

  • Hausberger M, Bruderer C, Le Scolan N, Pierre JS (2004) Interplay between environmental and genetic factors in temperament/personality traits in horses (Equus caballus). J Comp Psychol 118: 434-446

    Article  PubMed  Google Scholar 

  • Hausberger M, Muller C (2002) A brief note on some possible factors involved in the reactions of horses to humans. Appl Anim Behav Sci 76: 339-344

    Article  Google Scholar 

  • Inoue-Murayama M, Matsuura N, Murayama Y, Tsubota T, Iwasaki T, et al. (2002) Sequence comparison of the dopamine receptor D4 exon III repetitive region in several species of the order Carnivora. J Vet Med Sci 64: 747-749

    Article  PubMed  Google Scholar 

  • Ito H, Nara H, Inoue-Murayama M, Shimada MK, Koshimura A, et al. (2004) Allele frequency distribution of the canine dopamine receptor D4 gene Exon III and I in 23 breeds. J Vet Med Sci 66: 815-820

    Article  PubMed  Google Scholar 

  • Liu IS, Seeman P, Sanyal S, Ulpian C, Rodgers-Johnson PE, et al. (1996) Dopamine D4 receptor variant in Africans, D4valine194glycine, is insensitive to dopamine and clozapine: report of a homozygous individual. Am J Med Genet 61: 277-282

    Article  PubMed  Google Scholar 

  • Livak KJ, Rogers J, Lichter JB (1995) Variability of dopamine D4 receptor (DRD4) gene sequence within and among nonhuman primate species. Proc Natl Acad Sci U S A 92: 427-431

    PubMed  Google Scholar 

  • JM, Chandler C, Ball D (2001) Dopamine D4 receptor gene (DRD4) is associated with Novelty Seeking (NS) and substance abuse: the saga continues. Mol Psychiatry 6: 497-499

    Article  PubMed  Google Scholar 

  • Mader DR, Price EO (1980) Discrimination learning in horses: effects of breed, age and social dominance. J Anim Sci 50: 962-965

    PubMed  Google Scholar 

  • Momozawa Y, Kusunose R, Kikusui T, Takeuchi Y, Mori Y (2005) Assessment of equine temperament questionnaire by comparing factor structure between two separate surveys. Appl Anim Behav Sci 92, 77–84

    Article  Google Scholar 

  • Momozawa Y, Ono T, Sato F, Kikusui T, Takeuchi Y, et al. (2003) Assessment of equine temperament by a questionnaire survey to caretakers and evaluation of its reliability by simultaneous behavior test. Appl Anim Behav Sci 84: 127-138

    Article  Google Scholar 

  • Momozawa Y, Takeuchi Y, Tozaki T, Kikusui T, Hasegawa T, et al. (2005) Sequence, detection of polymorphisms and radiation hybrid mapping of the equine catechol-o-methyltransferase gene. Anim Genet 36: 190

    Article  PubMed  Google Scholar 

  • Nothen MM, Cichon S, Hemmer S, Hebebrand J, Remschmidt H, et al. (1994) Human dopamine D4 receptor gene: frequent occurrence of a null allele and observation of homozygosity. Hum Mol Genet 3: 2207-2212

    PubMed  Google Scholar 

  • O’Malley KL, Harmon S, Tang L, Todd RD (1992) The rat dopamine D4 receptor: sequence, gene structure, and demonstration of expression in the cardiovascular system. New Biol 4: 137-146

    PubMed  Google Scholar 

  • Paterson AD, Sunohara GA, Kennedy JL (1999) Dopamine D4 receptor gene: novelty or nonsense? Neuropsychopharmacology 21: 3-16

    Article  PubMed  Google Scholar 

  • Reif A, Lesch KP (2003) Toward a molecular architecture of personality. Behav Brain Res 139: 1-20

    Article  PubMed  Google Scholar 

  • Seeman P, Ulpian C, Chouinard G, Van Tol HH, Dwosh H, et al. (1994) Dopamine D4 receptor variant, D4GLYCINE194, in Africans, but not in Caucasians: no association with schizophrenia. Am J Med Genet 54: 384-390

    PubMed  Google Scholar 

  • Sondergaard E, Ladewig J (2004) Group housing exerts a positive effect on the behaviour of young horses during training. Appl Anim Behav Sci 87: 105-118

    Article  Google Scholar 

  • Van Tol HH, Bunzow JR, Guan HC, Sunahara RK, Seeman P, et al. (1991) Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine. Nature 350: 610-614

    Article  PubMed  Google Scholar 

  • Van Tol HH, Wu CM, Guan HC, Ohara K, Bunzow JR, et al. (1992) Multiple dopamine D4 receptor variants in the human population. Nature 358: 149-152

    Article  PubMed  Google Scholar 

  • Visser EK, van Reenen CG, Hopster H, Schilder MBH, Knaap JH, et al. (2001) Quantifying aspects of young horses’ temperament: consistency of behavioural variables. Appl Anim Behav Sci 74: 241-258

    Article  Google Scholar 

  • Visser EK, van Reenen CG, van der Werf JT, Schilder MB, Knaap JH, et al. (2002) Heart rate and heart rate variability during a novel object test and a handling test in young horses. Physiol Behav 76: 289-296

    Article  PubMed  Google Scholar 

  • Westberg L, Melke J, Landen M, Nilsson S, Baghaei F, et al. (2003) Association between a dinucleotide repeat polymorphism of the estrogen receptor alpha gene and personality traits in women. Mol Psychiatry 8: 118-122

    Article  PubMed  Google Scholar 

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Acknowledgment

The authors thank the staff of Hidaka Yearling Training Farm, Hokkaido (Japan Racing Association) for their kind help in this study. The authors are indebted to Dr. Telhisa Hasegawa of Equine Research Institute (Japan Racing Association) for instructing genetic analysis. This work was supported partially by a grant-in-aid for scientific research from the Ministry of Education, Science, Sports, Culture and Technology of Japan (No.17380167).

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Authors and Affiliations

  1. Laboratory of Veterinary Ethology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Yukihide Momozawa, Yukari Takeuchi, Takefumi Kikusui & Yuji Mori

  2. Equine Research Institute, Japan Racing Association, 321-4 Tokami-cho, Utsunomiya-shi, Tochigi, 320-0856, Japan

    Ryo Kusunose

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  1. Yukihide Momozawa
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  2. Yukari Takeuchi
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  3. Ryo Kusunose
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  4. Takefumi Kikusui
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  5. Yuji Mori
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Correspondence to Yukari Takeuchi.

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Momozawa, Y., Takeuchi, Y., Kusunose, R. et al. Association between equine temperament and polymorphisms in dopamine D4 receptor gene. Mamm Genome 16, 538–544 (2005). https://doi.org/10.1007/s00335-005-0021-3

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  • DOI: https://doi.org/10.1007/s00335-005-0021-3

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