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Identification and characterization of new microRNAs from pig

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Abstract

MicroRNAs (miRNAs) are small regulatory RNAs that direct the posttranscriptional repression of cognate messenger RNAs. Despite increasing evidence for diverse roles of miRNAs in biological processes, little is known about miRNAs in pig. We describe the first experimental identification of porcine miRNAs by sequence analysis of a cDNA library of small RNAs from porcine fibroblast cells. We identified 25 distinct porcine miRNAs, of which 19 are previously unreported, and define 14 new miRNA families in pig. Most of the cloned miRNAs are expressed ubiquitously in all porcine tissues examined, whereas some miRNAs are expressed preferentially in specific tissues. Our results enrich the porcine miRNA database and provide useful information for investigating biological functions of miRNAs in pig.

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Acknowledgments

This work was supported by a grant (20070301034036) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, South Korea

    Jung Kim, Ik Sang Cho, Jae Sang Hong, Hyunggee Kim & Young Sik Lee

  2. College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 361-763, South Korea

    Young Ki Choi

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  1. Jung Kim
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  2. Ik Sang Cho
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  5. Hyunggee Kim
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Correspondence to Young Sik Lee.

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Kim, J., Cho, I.S., Hong, J.S. et al. Identification and characterization of new microRNAs from pig. Mamm Genome 19, 570–580 (2008). https://doi.org/10.1007/s00335-008-9111-3

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