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Mapping of 22 expressed sequence tags isolated from a porcine small intestine cDNA library

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Abstract

Complementary DNA sequences were selected from a resource of tentatively identified clones from a porcine small intestine cDNA library. Forty PCR primer pairs were designed to amplify 101–309 base pairs of the 3′ untranslated region of the genes. The PCR conditions were optimized by altering both formamide and magnesium concentrations on samples of pig, mouse, and hamster DNA. Twenty primer pairs that, under stringent conditions, were pig-specific and amplified the expected fragments were chosen for regional assignment in a pig/rodent hybrid cell panel. Furthermore, 22 primer pairs were chosen to amplify DNA from the parental animals of the PiGMaP shared reference families in order to detect possible polymorphisms. Primer pairs that generated polymorphisms were used for genetic mapping. A total of 22 porcine expressed sequence tags (ESTs) were cytogenetically or genetically mapped by this approach. Twelve of the mapped ESTs could be added to the human-porcine comparative map.

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Authors and Affiliations

  1. Division of Animal Genetics, Department of Animal Science and Animal Health, The Royal Veterinary and Agricultural University, Bülowsvej 13, 1870 Frederiksberg C, Copenhagen, Denmark

    Claus B. Jørgensen, Anne Katrine Winterø & Merete Fredholm

  2. INRA, Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France

    Martine Yerle

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  1. Claus B. Jørgensen
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  2. Anne Katrine Winterø
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  3. Martine Yerle
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  4. Merete Fredholm
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Jørgensen, C.B., Winterø, A.K., Yerle, M. et al. Mapping of 22 expressed sequence tags isolated from a porcine small intestine cDNA library. Mammalian Genome 8, 423–427 (1997). https://doi.org/10.1007/s003359900460

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  • DOI: https://doi.org/10.1007/s003359900460

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