Abstract
We have used a PCR-based approach for the genetical and physical mapping of 34 transcripts isolated from a porcine small intestine cDNA library. All but one gene were regionally localized by using a somatic pig-rodent cell hybrid panel, and 12 genes were mapped by linkage analysis of single-stranded conformational polymorphisms developed in 3′ untranslated regions of transcripts. For 20 of the transcripts, the human homolog has already been mapped. This study thus represents a significant contribution to the pig comparative map. Some important findings were that we could clarify the extent of a previously identified inversion event in a region of conserved synteny between SSC6q and HSAlp, that SSC14q does contain a region homologous to HS Al, a situation not clear from earlier ZOO-FISH studies, and that the homology between SSCI7 and HSA20 includes the p-arm of HSA20.
Similar content being viewed by others
References
Adams MD, Jenny KM, Gocayne JD, Dubnick M, Polymeropoulos MH, Xiao H, Merril CR, Wu A, Olde B, Moreno RF, Kerlavage AR, McCombie RW, Venter CJ (1991) Complementary DNA sequencing: expressed sequence tags and human genome project. Science 252, 1651- 1656
Andersson L, Haley CS, Ellegren H, Knott SA, Johansson M, Andersson K, Andersson-Eklund L, Edfors-Lilja I, Fredholm M, Hansson I, Håkansson J, Lundström K (1994) Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263, 1771–1774
Archibald AL, Haley CS, Brown JF, Couperwhite HA, McQueen HA, Nicholoson D, Coppieters W, Van de Weghe A, Stratil A, Winterø AK, Fredholm M, Larsen NJ, Nielsen VH, Milan D, Woloszyn N, Robic A, Dalens M, Riquet J, Gellin J, Caritez J-C, Burgaud G, Ollivier L, Bidanel J-P, Vaiman M, Renard C, Geldermann H, Davoli R, Ruyter D, Verstege EJM, Groenen MAM, Davies W, Høyheim B, Keiserud A, Andersson L, Ellegren H, Johansson M, Marklund L, Miller JR, Anderson Dear DV, Signer E, Jeffreys AJ, Moran C, Le Tissier P, Muladno, Rothschild MF, Tuggle CK, Vaske D, Helm J, Liu H-C, Rahman A, Yu T-P, Larson RG, Schmitz CB (1995) The PiGMap consortium linkage map of the pig (Sus scrofa). Mamm Genome 6, 157–175
Beier DR, Dushkin H, Sussman DJ (1992) Mapping genes in the mouse using single-strand conformation polymorphism analysis of recombinant inbred strains and interspecific crosses. Proc Natl Acad Sci USA 89, 9102–9106
Chevalet C, Gouzy J, San Cristobal-Gaudy M (1996) Regional assignment of genetic markers using a somatic cell hybrid panel: a WWW interactive program available for the pig genome. Comput Appl Biosci in press
Ellegren H, Fredholm M, Edfors-Lilja I, Winterø AK, Andersson L (1993) Conserved synteny between pig chromosome 8 and human chromosome 4 but rearranged and distorted linkage maps. Genomics 17, 599–603
Ellegren H, Chowdhary BP, Johansson M, Marklund L, Fredholm M, Gustavsson I, Andersson L (1994) A primary linkage map of the porcine genome reveals a low rate of genetic recombination in the pig. Genetics 137, 1089–1100
Frönicke L, Chowdhary BP, Schertan H, Gustavsson I (1996) A comparative map of the porcine and human genomes demonstrates ZOO-FISH and gene mapping-based chromosomal homologies. Mamm Genome 7, 285–290
Gish W, States DJ (1993) Identification of protein coding regions by database similarity search. Nature Genet 3, 266–272
Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat G, Gellin J (1996) Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36, 252- 262
Green P, Falls KA, Crooks S (1990) Documentation for CRIMAP, version 2.4. (St Louis, Mo.: Washington Univ. School of Medicine)
Hasler-Rapacz J, Ellegren H, Fridolfsson A-K, Kirkpatrick B, Scott K, Andersson L, Rapacz J (1997) Positional candidate cloning of a gene for familial hypercholesterolemia in swine. Am J Med Genet in press.
Johansson M, Ellegren H, Andersson L (1995) Comparative mapping reveals extensive linkage conservation—but with gene order rearrangements—between the pig and the human genomes. Genomics 25, 682- 690
Leeb T, Rettenberger G, Hameister H, Brem G, Brenig B (1995) Construction of a porcine YAC library and mapping of the cardiac-muscle ryanodine receptor gene to chromosome 14q22-q23. Mamm Genome 6, 37–11
Li W-H, Graur D (1991) Fundamentals of Molecular Evolution. (Sunderland, Mass.: Sinauer Associates)
Mariani P, Lundström K, Gustafsson U, Enfält A-C, Juneja RK, Andersson L (1996) A major locus (RN) affecting muscle glycogen content is located on pig chromosome 15. Mamm Genome 7, 52–54
Marklund L, Johansson-Moller M, Høyheim B, Davies W, Fredholm M, Juneja RK, Mariani P, Coppieters W, Ellegren H, Andersson L (1996) A comprehensive linkage map of the pig based on a wild pig-Large White intercross. Anim Genet 17, 255–269
Milan D, Woloszyn N, Yerle M, Le Roy P, Bonnet M, Riquet J, Lahbib-Mansais Y, Caritez J-C, Robic A, Sellier P, Elsen J-M, Gellin J (1996) Accurate mapping of the “acid meat” RN gene on genetic and physical maps of pig chromosome 15. Mamm Genome 7, 47–52
Nunes M, Yerle M, Dezeure F, Gellin J, Chardon P, Vaiman M (1993) Isolation of four HSP70 genes in pig and localization of Chromosomes 7 and 14. Mamm Genome 4, 247–251
Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85, 2444–2448
Polymeropoulos MH, Xiao H, Sikela JM, Adams M, Venter CJ, Merril CR (1993) Chromosomal distribution of 320 genes from a brain cDNA library. Nature Genet 4, 381–386
Rettenberger G, Klett C, Zechner U, Kunz J, Vogel W, Hameister H (1995) Visualization of the conservation of synteny between humans and pigs by heterologous chromosomal painting. Genomics 26, 372–378
Robic A, Riquet J, Yerle M, Milan D, Lahbib-Mansais Y, Dubot-Fontana C, Gellin J (1996) Porcine linkage and cytogenetic maps integrated by regional mapping of 100 microsatellites on somatic cell hybrid panel. Mamm Genome 7, 438–445
Rohrer GA, Alexander LJ, Hu Z, Smith TPL, Keele JW, Beattie CW (1996) A comprehensive map of the porcine genome. Genome Res 6, 371–391
Schuler GD, Boguski MS, Stewart EA, Stein LD, Gyapay G, Rice K, White RE, Rodriguez-Tomé P, Aggarwal A, Bajorek E, Bentolila S, Birren BB, Butler A, Castle AB, Chiannilkulchai N, Chu A, Clee C, Cowles S, Day PJR, Dibling T, Drouot N, Dunham I, Duprat S, East C, Edwards C, Fan J-B, Fang N, Fizames C, Garrett C, Green L, Hadley D, Harris M, Harrison P, Brady S, Hicks A, Holloway E, Hui L, Hussain S, Louis-Dit-Sully C, Ma J, MacGilvery A, Mader C, Maratukulam A, Matise TC, McKusick KB, Morissette J, Mungall A, Muselet D, Nusbaum HC, Page DC, Peck A, Perkins S, Piercy M, Qin F, Quackenbush J, Ranby S, Reif T, Rozen S, Sanders C, She X, Silva J, Slonim DK, Soderlund C, Sun W-L, Tabar P, Thangarajah T, Vega-Czarny N, Vollrath D, Voyticky S, Wilmer T, Wu X, Adams MD, Auffray C, Walter NAR, Brandon R, Dehejia A, Goodfellow PN, Houlgatte R, Hudson Jr JR, Ide SE, Iorio KR, Lee WY, Seki N, Nagase T, Ishikawa K, Nomura N, Phillips C, Polymeropoulos MH, Sandusky M, Schmitt K, Berry R, Swanson K, Torres R, Venter JC, Sikela JM, Beckmann JS, Weissenbach J, Myers RM, Cox DR, James MR, Bentley D, Deloukas P, Lander ES, Hudson TJ (1996) A gene map of the human genome. Science 274, 540–546
Wilcox AS, Khan AS, Hopkins JA, Sikela JM (1991) Use of 3′untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implications for an expression map of the genome. Nucleic Acids Res. 19, 1837–1843
Winterø AK, Fredholm M, Davies W (1996) Evaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones. Mamm Genome 7, 509–517
Womack JE, Kata SR (1995) Bovine genome mapping: evolutionary inference and the power of comparative genomics. Curr Opin Genet Dev 5, 725–733
Yerle M, Lahbib-Mansais Y, Mellink C, Goureau A, Pinton P, Echard G, Gellin J, Schmitz A, Chaput B, Frelat G, Zijstra C, De Haan, Christensen K, Rettenberger G, Hameister H (1995) The PiGMap Consortium cytogenetic map of the domestic pig (Sus scrofa domestica). Mamm Genome 6, 176–186
Yerle M, Echard G, Robic A, Mairal A, Dubot-Fontana C, Riquet J, Pinton P, Milan D, Lahbib-Hansais Y, Gellin J (1996) A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet Cell Genet 73, 194–202
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fridolfsson, A.K., Hori, T., Winterø, A.K. et al. Expansion of the pig comparative map by expressed sequence tags (EST) mapping. Mammalian Genome 8, 907–912 (1997). https://doi.org/10.1007/s003359900609
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003359900609