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Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia

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Abstract

Schizophrenia is a complex disease, likely to be caused by a combination of serial alterations in a number of genes and environmental factors. The dorsolateral prefrontal cortex (Brodmann's Area 46) is involved in schizophrenia and executes high-level functions such as working memory, differentiation of conflicting thoughts, determination of right and wrong concepts and attitudes, correct social behavior and personality expression. Global proteomic analysis of post-mortem dorsolateral prefrontal cortex samples from schizophrenia patients and non-schizophrenic individuals was performed using stable isotope labeling and shotgun proteomics. The analysis resulted in the identification of 1,261 proteins, 84 of which showed statistically significant differential expression, reinforcing previous data supporting the involvement of the immune system, calcium homeostasis, cytoskeleton assembly, and energy metabolism in schizophrenia. In addition a number of new potential markers were found that may contribute to the understanding of the pathogenesis of this complex disease.

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Acknowledgments

The authors would like to thank ABADHS (Associação Beneficente Alzira Denise Hertzog da Silva), FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo), and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for the financial support of this project in Brazil. We also would like to acknowledge the DAAD (Deutscher Akademischer Austauschdienst) and the Max Planck Society for their financial support of this work in Germany. We thank Ms. Molly McEwen for editorial help.

Author information

Author notes

  1. Emmanuel Dias-Neto

    Present address: University of Texas, MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX, 77030, USA

Authors and Affiliations

  1. Laboratório de Neurociências, Instituto de Psiquiatria-Faculdade de Medicina-Universidade de São Paulo, Rua. Dr. Ovidio Pires de Campos, no 785, Consolação, São Paulo, SP, 05403-010, Brazil

    Daniel Martins-de-Souza, Wagner F. Gattaz & Emmanuel Dias-Neto

  2. Max Planck Institute of Psychiatry, Kraepelinstrasse 2, 80804, Munich, Germany

    Daniel Martins-de-Souza, Christiane Rewerts, Giuseppina Maccarrone & Christoph W. Turck

  3. Department of Psychiatry, University of Goettingen, Von Siebold Str. 5, 37075, Goettingen, Germany

    Andrea Schmitt

  4. Central Institute of Mental Health, J5, 68159, Mannheim, Germany

    Andrea Schmitt

Authors
  1. Daniel Martins-de-Souza
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  2. Wagner F. Gattaz
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  3. Andrea Schmitt
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  4. Christiane Rewerts
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  5. Giuseppina Maccarrone
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  6. Emmanuel Dias-Neto
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  7. Christoph W. Turck
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Corresponding authors

Correspondence to Emmanuel Dias-Neto or Christoph W. Turck.

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Martins-de-Souza, D., Gattaz, W.F., Schmitt, A. et al. Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia. Eur Arch Psychiatry Clin Neurosci 259, 151–163 (2009). https://doi.org/10.1007/s00406-008-0847-2

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  • DOI: https://doi.org/10.1007/s00406-008-0847-2

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