Abstract
This paper presents the allele frequency distributions for the nine loci (D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820) present in the commercially available Profiler Plus kit. DNA samples of 202 individuals from Northern Poland were amplified in a multiplex reaction with subsequent automatic detection using capillary electrophoresis. All loci met the Hardy-Weinberg equilibrium conditions. The calculated probability of identity was 2.26 × 10–11 giving an average probability of identity of 1 in 44 billion. Considerable improvement of analysis precision was observed after substituting the GeneScan 500 for the fluorescent ladder 60–400 bp. The influence of DNA concentration on stutter and artefact formation and the ratio of heterozygote alleles was analysed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 15 September 1999 / Accepted: 14 January 2000
Rights and permissions
About this article
Cite this article
Pawlowski, R., Maciejewska, A. Forensic validation of a multiplex containing nine STRs – population genetics in Northern Poland. Int J Leg Med 114, 45–49 (2000). https://doi.org/10.1007/s004140000139
Issue Date:
DOI: https://doi.org/10.1007/s004140000139