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Expression of TRPC homologs in endothelial cells and smooth muscle layers of human arteries

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Abstract

TRPC channels are a group of Ca2+-permeable nonselective cation channels that mediate store-operated and/or agonist-stimulated Ca2+ influx in a variety of cell types. In this study, we extensively examined the expression patterns of TRPC homologs in human vascular tissues. RT-PCR amplified cDNA fragments of TRPC1 (505 bp), TRPC3 (372 bp), TRPC4 (499 bp), TRPC5 (325 bp), TRPC6 (509 bp), and TRPC7 (187 bp) from RNA isolated from cultured human coronary artery endothelial cells. In situ hybridization yielded strong labeling of TRPC1,3–6 in the endothelial and smooth muscle cells of human coronary and cerebral arteries. TRPC7 labeling was exclusively found in endothelial cells but not in smooth muscle cells. Results from immunohistochemical staining were consistent with those from in situ hybridization. Similar expression patterns of TRPC homologs were also observed in arterioles and vaso vasora. In conclusion, our study indicates that TRPC homologs are widely expressed in human vessels of all calibers, including medium-sized coronary arteries and cerebral arteries, smaller-sized resistance arteries, and vaso vasora. These results suggest a ubiquitous role of TRPC homologs in regulating blood supply to different regions and in controlling arterial blood pressure.

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Acknowledgements

This study was supported by Hong Kong Research Grant Council (CUHK4174/02 M) and France/Hong Kong Joint Research Scheme (F-HK11/01T).

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong, China

    Ham Yip, Pan-Cheung Leung, Hiu-Yee Kwan, Cuiling Liu, Yu Huang & Xiaoqiang Yao

  2. Department of Anatomical and Cellular Pathology, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong, China

    Wing-Yee Chan

  3. Laboratorie Canaux Ioniques et Signalisation, Department de Biologie Moleculaire et Structurale, INSERM E9931, 17 Rue des Martyrs, 38054, Grenoble, France

    Villaz Michel

  4. Department of Anatomy, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong, China

    David Tai-Wai Yew

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  1. Ham Yip
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  2. Wing-Yee Chan
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  4. Hiu-Yee Kwan
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  8. David Tai-Wai Yew
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  9. Xiaoqiang Yao
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Correspondence to Xiaoqiang Yao.

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Yip, H., Chan, WY., Leung, PC. et al. Expression of TRPC homologs in endothelial cells and smooth muscle layers of human arteries. Histochem Cell Biol 122, 553–561 (2004). https://doi.org/10.1007/s00418-004-0720-y

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  • DOI: https://doi.org/10.1007/s00418-004-0720-y

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