Early metabolic adaptations of rabbit fast-twitch muscle to chronic low-frequency stimulation

Abstract

To investigate early adaptive responses to chronic low-frequency stimulation (CLFS), rabbit tibialis anterior (TA) muscles were continuously stimulated at 10 Hz for 8 days, allowed to rest for 1 h, and then subjected to a 15-min fatigue test at 10-Hz stimulation. The contralateral TA muscles which had not been exposed to CLFS, served as controls during the fatigue test. Compared to the controls, the initial tension output of the 8-day prestimulated muscles was reduced by 25%. However, these muscles maintained higher tensions during the fatigue test than the controls. Citrate synthase activity, an indicator of aerobic-oxidative capacity, was only slightly elevated (40%) in the 8-day stimulated muscles. Unlike the controls, the prestimulated muscles failed to produce potentiation during the fatigue test. Control muscles responded to the fatigue test with pronounced reductions in contents of adenosine 5′-triphosphate (ATP), phosphocreatine (PCr), and glycogen, as well as with large increases in contents of inosine monophosphate (IMP), inorganic phosphate (P_i), creatine (Cr), and lactate. Under the same conditions contents of ATP, PCr, Cr, glycogen, lactate, P_i, and IMP were unaltered in the 8-day prestimulated muscles. These findings demonstrated that CLFS for 8 days elicited pronounced alterations in energy metabolism and contractile properties. These adaptive changes occurred prior to fibre type transitions and substantial increases in aerobic-oxidative potential.