Skip to main content
SpringerLink
Log in

Effects of different fatty acids and dietary lipids on adiponectin gene expression in 3T3-L1 cells and C57BL/6J mice adipose tissue

  • Endocrinology
  • Published:
Pflügers Archiv - European Journal of Physiology Aims and scope Submit manuscript

Abstract

Obesity is positively correlated to dietary lipid intake, and the type of lipid may play a causal role in the development of obesity-related pathologies. A major protein secreted by adipose tissue is adiponectin, which has antiatherogenic and antidiabetic properties. The aim of this study was to evaluate the effects of four different high-fat diets (enriched with soybean oil, fish oil, coconut oil, or lard) on adiponectin gene expression and secretion by the white adipose tissue (WAT) of mice fed on a selected diet for either 2 (acute treatment) or 60 days (chronic treatment). Additionally, 3T3-L1 adipocytes were treated for 48 h with six different fatty acids: palmitic, linoleic, eicosapentaenoic (EPA), docosahexaenoic (DHA), lauric, or oleic acid. Serum adiponectin concentration was reduced in the soybean-, coconut-, and lard-enriched diets in both groups. Adiponectin gene expression was lower in retroperitoneal WAT after acute treatment with all diets. The same reduction in levels of adiponectin gene expression was observed in epididymal adipose tissue of animals chronically fed soybean and coconut diets and in 3T3-L1 cells treated with palmitic, linoleic, EPA, and DHA acids. These results indicate that the intake of certain fatty acids may affect serum adiponectin levels in mice and adiponectin gene expression in mouse WAT and 3T3-L1 adipocytes. The effects appear to be time dependent and depot specific. It is postulated that the downregulation of adiponectin expression by dietary enrichment with soybean oil or coconut oil may contribute to the development of insulin resistance and atherosclerosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Ajuwon KM, Spurlock ME (2005) Palmitate activates the NF-kappaB transcription factor and induces IL-6 and TNFalpha expression in 3T3-L1 adipocytes. J Nutr 135:1841–1846

    PubMed  CAS  Google Scholar 

  2. Arita Y, Kihara S, Ouchi N et al (1999) Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun 257:79–83

    Article  PubMed  CAS  Google Scholar 

  3. Bhattacharya A, Lawrence RA, Krishnan A, Zaman K, Sun D, Fernandes G (2003) Effect of dietary n-3 and n-6 oils with and without food restriction on activity of antioxidant enzymes and lipid peroxidation in livers of cyclophosphamide treated autoimmune-prone NZB/W female mice. J Am Coll Nutr 22:388–399

    PubMed  CAS  Google Scholar 

  4. Boozer CN, Schoenbach G, Atkinson RL (1995) Dietary fat and adiposity: a dose–response relationship in adult male rats fed isocalorically. Am J Physiol 268:E546–E550

    PubMed  CAS  Google Scholar 

  5. Bruun JM, Lihn AS, Verdich C, Pedersen SB, Toubro S, Astrup A, Richelsen B (2003) Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab 285:E527–E533

    PubMed  CAS  Google Scholar 

  6. Cha MC, Jones PJ (1998) Dietary fat type and energy restriction interactively influence plasma leptin concentration in rats. J Lipid Res 39:1655–1660

    PubMed  CAS  Google Scholar 

  7. Crozier G, Bois-Joyeux B, Chanez M, Girard J, Peret J (1987) Metabolic effects induced by long-term feeding of medium-chain triglycerides in the rat. Metabolism 36:807–814

    Article  PubMed  CAS  Google Scholar 

  8. Degawa-Yamauchi M, Moss KA, Bovenkerk JE, Shankar SS, Morrison CL, Lelliott CJ, Vidal-Puig A, Jones R, Considine RV (2005) Regulation of adiponectin expression in human adipocytes: effects of adiposity, glucocorticoids, and tumor necrosis factor alpha. Obes Res 13:662–669

    PubMed  CAS  Google Scholar 

  9. Diez JJ, Iglesias P (2003) The role of the novel adipocyte-derived hormone adiponectin in human disease. Eur J Endocrinol 148:293–300

    Article  PubMed  CAS  Google Scholar 

  10. Dube MG, Beretta E, Dhillon H, Ueno N, Kalra PS, Kalra SP (2002) Central leptin gene therapy blocks high-fat diet-induced weight gain, hyperleptinemia, and hyperinsulinemia: increase in serum ghrelin levels. Diabetes 51(6):1729–1736 Jun

    Article  PubMed  CAS  Google Scholar 

  11. Fasshauer M, Klein J, Neumann S, Eszlinger M, Paschke R (2002) Hormonal regulation of adiponectin gene expression in 3T3-L1 adipocytes. Biochem Biophys Res Commun 90:1084–1089

    Article  CAS  Google Scholar 

  12. Flachs P, Mohamed-Ali V, Horakova O, Rossmeisl M, Hosseinzadeh-Attar MJ, Hensler M, Ruzickova J, Kopecky J (2006) Polyunsaturated fatty acids of marine origin induce adiponectin in mice fed a high-fat diet. Diabetologia 49:394–397

    Article  PubMed  CAS  Google Scholar 

  13. Gaiva MH, Couto RC, Oyama LM, Couto GE, Silveira VL, Ribeiro EB, Nascimento CM (2001) Polyunsaturated fatty acid-rich diets: effect on adipose tissue metabolism in rats. Br J Nutr 86:371–377

    PubMed  CAS  Google Scholar 

  14. Garland M, Sacks FM, Colditz GA, Rimm EB, Sampson LA, Willett WC, Hunter DJ (1998) The relation between dietary intake and adipose tissue composition of selected fatty acids in US women. Am J Clin Nutr 67:25–30

    PubMed  CAS  Google Scholar 

  15. Gonzalez MJ, Gray JI, Schemmel RA, Dugan L Jr, Welsch CW (1992) Lipid peroxidation products are elevated in fish oil diets even in the presence of added antioxidants. J Nutr 122:2190–2195

    PubMed  CAS  Google Scholar 

  16. Haugen F, Jorgensen A, Drevon CA, Trayhurn P (2001) Inhibition by insulin of resistin gene expression in 3T3-L1 adipocytes. FEBS Lett 507:105–108

    Article  PubMed  CAS  Google Scholar 

  17. Himaya A, Fantino M, Antoine JM, Brondel L, Louis-Sylvestre J (1997) Satiety power of dietary fat: a new appraisal. Am J Clin Nutr 65:1410–1418

    PubMed  CAS  Google Scholar 

  18. Hotta K, Funahashi T, Arita Y et al (2000) Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol 20:1595–1599

    PubMed  CAS  Google Scholar 

  19. Hu FB, Van Dam RM, Liu S (2001) Diet and risk of Type II diabetes: the role of types of fat and carbohydrate. Diabetologia 44:805–817

    Article  PubMed  CAS  Google Scholar 

  20. Jain SK, Kannan K, Lim G, McVie R, Bocchini JA Jr (2002) Hyperketonemia increases tumor necrosis factor-alpha secretion in cultured U937 monocytes and Type 1 diabetic patients and is apparently mediated by oxidative stress and cAMP deficiency. Diabetes 51:2287–2293

    Article  PubMed  CAS  Google Scholar 

  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  22. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K (1996) cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1). Biochem Biophys Res Commun 221:286–289

    Article  PubMed  CAS  Google Scholar 

  23. Maggio CA, Haraczkiewicz E, Vasselli JR (1988) Diet composition alters the satiety effect of cholecystokinin in lean and obese Zucker rats. Physiol Behav 43:485–491

    Article  PubMed  CAS  Google Scholar 

  24. Milan G, Granzotto M, Scarda A, Calcagno A, Pagano C, Federspil G, Vettor R (2002) Resistin and adiponectin expression in visceral fat of obese rats: effect of weight loss. Obes Res 10:1095–1103

    Article  PubMed  CAS  Google Scholar 

  25. Neschen S, Morino K, Rossbacher JC, Pongratz RL, Cline GW, Sono S, Gillum M, Shulman GI (2006) Fish oil regulates adiponectin secretion by a peroxisome proliferator-activated receptor-gamma-dependent mechanism in mice. Diabetes 55:924–928

    Article  PubMed  CAS  Google Scholar 

  26. Oller do Nascimento CM, Williamson DH (1988) Tissue-specific effects of starvation and refeeding on the disposal of oral [1–14C]triolein in the rat during lactation and on removal of litter. Biochem J 254:539–546

    PubMed  CAS  Google Scholar 

  27. Oudart H, Groscolas R, Calgari C, Nibbelink M, Leray C, Le Maho Y, Malan A (1997) Brown fat thermogenesis in rats fed high-fat diets enriched with n-3 polyunsaturated fatty acids. Int J Obes Relat Metab Disord 21:955–962

    Article  PubMed  CAS  Google Scholar 

  28. Perez-Matute P, Marti A, Martinez JA, Fernandez-Otero MP, Stanhope KL, Havel PJ, Moreno-Aliaga MJ (2005) Eicosapentaenoic fatty acid increases leptin secretion from primary cultured rat adipocytes: role of glucose metabolism. Am J Physiol Regul Integr Comp Physiol 288:R1682–R1688

    PubMed  CAS  Google Scholar 

  29. Rayner DV, Miller S (1993) Voluntary intake and gastric emptying in pigs: effects of fat and a CCK inhibitor. Physiol Behav 54:917–922

    Article  PubMed  CAS  Google Scholar 

  30. Sacks FM, Katan M (2002) Randomized clinical trials on the effects of dietary fat and carbohydrate on plasma lipoproteins and cardiovascular disease. Am J Med 113:13S–24S

    Article  PubMed  CAS  Google Scholar 

  31. Soares AF, Guichardant M, Cozzone D, Bernoud-Hubac N, Bouzaidi-Tiali N, Lagarde M, Geloen A (2005) Effects of oxidative stress on adiponectin secretion and lactate production in 3T3-L1 adipocytes. Free Radic Biol Med 38:882–889

    Article  PubMed  CAS  Google Scholar 

  32. Stansbie D, Brownsey RW, Crettaz M, Denton RM (1976) Acute effects in vivo of anti-insulin serum on rates of fatty acid synthesis and activities of acetyl–coenzyme A carboxylase and pyruvate dehydrogenase in liver and epididymal adipose tissue of fed rats. Biochem J 160:413–416

    PubMed  CAS  Google Scholar 

  33. Su W, Jones PJ (1993) Dietary fatty acid composition influences energy accretion in rats. J Nutr 123:2109–2114

    PubMed  CAS  Google Scholar 

  34. Takahashi Y, Ide T (2000) Dietary n-3 fatty acids affect mRNA level of brown adipose tissue uncoupling protein 1, and white adipose tissue leptin and glucose transporter 4 in the rat. Br J Nutr 84:175–184

    PubMed  CAS  Google Scholar 

  35. Todoric J, Loffler M, Huber J, Bilban M, Reimers M, Kadl A, Zeyda M, Waldhausl W, Stulnig TM (2006) Adipose tissue inflammation induced by high-fat diet in obese diabetic mice is prevented by n-3 polyunsaturated fatty acids. Diabetologia 49:2109–2119

    Article  PubMed  CAS  Google Scholar 

  36. Trayhurn P, Duncan JS, Nestor A, Thomas ME, Rayner DV (1994) Chemiluminescent detection of mRNAs on northern blots with digoxigenin end-labeled oligonucleotides. Anal Biochem 222:224–230

    Article  PubMed  CAS  Google Scholar 

  37. Trayhurn P, Thomas ME, Duncan JS, Rayner DV (1995) Effects of fasting and refeeding on ob gene expression in white adipose tissue of lean and obese (oblob) mice. FEBS Lett 368:488–490

    Article  PubMed  CAS  Google Scholar 

  38. Wang B, Jenkins JR, Trayhurn P (2005) Expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture: integrated response to TNF-alpha. Am J Physiol Endocrinol Metab 288:E731–E740

    Article  PubMed  CAS  Google Scholar 

  39. Warensjo E, Sundstrom J, Lind L, Vessby B (2006) Factor analysis of fatty acids in serum lipids as a measure of dietary fat quality in relation to the metabolic syndrome in men. Am J Clin Nutr 84:442–448

    PubMed  Google Scholar 

  40. West DB, York B (1998) Dietary fat, genetic predisposition, and obesity: lessons from animal models. Am J Clin Nutr 67:505S–512S

    PubMed  CAS  Google Scholar 

  41. Wohlers M, Nascimento CM, Xavier RA, Ribeiro EB, Silveira VL (2003) Participation of corticosteroids and effects of indomethacin on the acute inflammatory response of rats fed n-6 or n-3 polyunsaturated fatty acid-rich diets. Inflammation 27:1–7

    Article  PubMed  CAS  Google Scholar 

  42. Wohlers M, Xavier RA, Oyama LM, Ribeiro EB, do Nascimento CM, Casarini DE, Silveira VL (2005) Effect of fish or soybean oil-rich diets on bradykinin, kallikrein, nitric oxide, leptin, corticosterone and macrophages in carrageenan stimulated rats. Inflammation 29:81–89

    Article  PubMed  CAS  Google Scholar 

  43. Yamamoto Y, Hirose H, Saito I, Tomita M, Taniyama M, Matsubara K, Okazaki Y, Ishii T, Nishikai K, Saruta T (2002) Correlation of the adipocyte-derived protein adiponectin with insulin resistance index and serum high-density lipoprotein-cholesterol, independent of body mass index, in the Japanese population. Clin Sci (Lond) 103:137–142

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo). The authors thank Mr. Caio Sussumu Motoyama and Mrs. Carolina Biz for technical assistance. The first author thanks Dr. Muhammad Peeraully and Dr. Vivien Hartley Ryen for reviewing the text.

Conflict of interest

The authors of this research disclose any potential conflict of interest.

Author information

Authors and Affiliations

  1. Department of Physiology, Campus Sao Paulo, São Paulo Federal University, São Paulo, Brazil

    Allain Amador Bueno, Cristiane de Oliveira, Luciana Pelegrini Pisani, Eliane Beraldi Ribeiro & Cláudia Maria Oller do Nascimento

  2. Department of Health Sciences, Campus Baixada Santista, São Paulo Federal University, São Paulo, Brazil

    Lila Missae Oyama

  3. Department of Biology, Campus Diadema, São Paulo Federal University, São Paulo, Brazil

    Vera Lucia Flor Silveira

  4. Departamento de Fisiologia—Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu, 862, 2° andar—Edificio de Ciências Biomédicas, São Paulo, SP, 04023060, Brazil

    Cláudia Maria Oller do Nascimento

Authors
  1. Allain Amador Bueno
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lila Missae Oyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cristiane de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Luciana Pelegrini Pisani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eliane Beraldi Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vera Lucia Flor Silveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Cláudia Maria Oller do Nascimento
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cláudia Maria Oller do Nascimento.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bueno, A.A., Oyama, L.M., de Oliveira, C. et al. Effects of different fatty acids and dietary lipids on adiponectin gene expression in 3T3-L1 cells and C57BL/6J mice adipose tissue. Pflugers Arch - Eur J Physiol 455, 701–709 (2008). https://doi.org/10.1007/s00424-007-0330-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00424-007-0330-3

Keywords

Search

Navigation