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Functional TRPV4 channels are expressed in mouse skeletal muscle and can modulate resting Ca2+ influx and muscle fatigue

  • Muscle Physiology
  • Published:
Pflügers Archiv - European Journal of Physiology Aims and scope Submit manuscript

Abstract

Skeletal muscle contraction is basically controlled by Ca2+ release and its reuptake into the sarcoplasmic reticulum. However, the long-term maintenance of muscle function requires an additional Ca2+ influx from extracellular. Several mechanisms seem to contribute to the latter process, such as store-operated Ca2+ entry, stretch-activated Ca2+ influx and resting Ca2+ influx. Candidate channels that may control Ca2+ influx into muscle fibers are the STIM proteins, Orai, and the members of the transient receptor potential (TRP) family of cation channels. Here we show that TRPV4, an osmo-sensitive cation channel of the vanilloid subfamily of TRP channels is functionally expressed in mouse skeletal muscle. Western blot analysis showed the presence of TRPV4-specific bands at about 85 and 100 kDa in all tested muscles. The bands were absent when muscle proteins from TRPV4 deficient mice were analyzed. Using the manganese quench technique, we studied the resting influx of divalent cations into isolated wild-type muscle fibers. The specific TRPV4-channel activator 4α-phorbol-12,13-didecanoate (4α-PDD) stimulated resting influx by about 60% only in wild-type fibers. Electrical stimulation of soleus muscles did not reveal changes in isometric twitch contractions upon application of 4α-PDD, but tetanic contractions (at 120 Hz) were slightly increased by about 15%. When soleus muscles were stimulated with a fatigue protocol, muscle fatigue was significantly attenuated in the presence of 4α-PDD. The latter effect was not observed with muscles from TRPV4−/− mice. We conclude that TRPV4 is functionally expressed in mouse skeletal muscle and that TRPV4 activation modulates resting Ca2+ influx and muscle fatigue.

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Acknowledgement

We thank Dr. R. Köhler for providing us TRPV4−/− mice. We are grateful to Ms. D. Schulz and Ms. H. Kenk for excellence technical assistance. This work was supported by the European Union (proposal No. 229750) and aktion benni & Co e.V.

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Authors and Affiliations

  1. Institute of Pathophysiology, University of Greifswald, Greifswald, Germany

    Bernd W. Pritschow, Thom Lange, Joachim Kasch, Christiane Kunert-Keil & Heinrich Brinkmeier

  2. Center for Translational Neuroscience, Duke University, Durham, NC, USA

    Wolfgang Liedtke

  3. Institute of Pathophysiology, University of Greifswald, Greifswalder Str. 11C, 17495, Karlsburg, Germany

    Heinrich Brinkmeier

Authors
  1. Bernd W. Pritschow
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  2. Thom Lange
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  3. Joachim Kasch
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  4. Christiane Kunert-Keil
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  5. Wolfgang Liedtke
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  6. Heinrich Brinkmeier
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Correspondence to Heinrich Brinkmeier.

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Bernd W. Pritschow and Thom Lange contributed equally to this work.

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Pritschow, B.W., Lange, T., Kasch, J. et al. Functional TRPV4 channels are expressed in mouse skeletal muscle and can modulate resting Ca2+ influx and muscle fatigue. Pflugers Arch - Eur J Physiol 461, 115–122 (2011). https://doi.org/10.1007/s00424-010-0883-4

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  • DOI: https://doi.org/10.1007/s00424-010-0883-4

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