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Firewall function of the endothelial glycocalyx in the regulation of sodium homeostasis

  • Cardiovascular Physiology
  • Published:
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Abstract

Plasma sodium, slightly above normal and in presence of aldosterone, stiffens vascular endothelium and reduces nitric oxide release with the consequence of endothelial dysfunction. This process is mediated by epithelial sodium channels (ENaC) and, most likely, the endothelial Na+/K+-ATPase. Both, ENaC and Na+/K+-ATPase, are located in the plasma membrane of endothelial cells and embedded in the endothelial glycocalyx (eGC). This negatively charged biopolymer is directly exposed to the blood stream and selectively buffers sodium ions. We hypothesize that the glycocalyx could interfere with endothelial sodium transport when extracellular sodium varies in the physiological range. Therefore, we modeled the endothelial cell as a pump–leak system measuring changes of intracellular sodium in cultured human endothelial cells. Experiments were performed under low/high extracellular sodium conditions before and after enzymatic eGC removal, and with inhibition of Na+/K+-ATPase and ENaC, respectively. Three major observations were made: (1) eGC removal by heparinase treatment facilitates sodium to enter/exit the endothelial cells. (2) The direction of net sodium movement across the endothelial plasma membrane depends on the concentration of extracellular sodium which regulates both the Na+/K+-ATPase and ENaC activity. (3) Removal of eGC and inhibition of sodium transport modify the electrical resistance of endothelial cells. We conclude that the eGC serves as a potential “firewall” preventing uncontrolled access of sodium to the pump–leak system of the endothelial cell. After eGC removal, sodium access to the system is facilitated. Thus the pump–leak system could be regulated by ambient sodium and control vascular permeability in pathophysiological conditions.

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Acknowledgements

This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, OB 63/17-1 and Koselleck-OB 63/18), the University of Münster (thesis scholarship to S.K.), and the Innovative Medizinische Forschung (IMF), KO 620904. The authors would like to thank COST Action TD1002 for supporting their networking activities.

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Authors and Affiliations

  1. Institute of Physiology II, University of Muenster, Robert-Koch-Strasse 27 b, 48149, Münster, Germany

    Stefanie Korte, Alexandra S. Straeter, Wladimir Peters, Hans Oberleithner & Kristina Kusche-Vihrog

  2. Department of Internal Medicine D, University Hospital Muenster, Muenster, Germany

    Anne Wiesinger

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  1. Stefanie Korte
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Correspondence to Kristina Kusche-Vihrog.

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Korte, S., Wiesinger, A., Straeter, A.S. et al. Firewall function of the endothelial glycocalyx in the regulation of sodium homeostasis. Pflugers Arch - Eur J Physiol 463, 269–278 (2012). https://doi.org/10.1007/s00424-011-1038-y

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  • DOI: https://doi.org/10.1007/s00424-011-1038-y

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