Abstract
Recently, the cell-volume-regulated serine-threonine protein kinase h-sgk was cloned from a human hepatoma cell line. The sgk gene was shown to be induced by cell shrinkage in many different mammalian cell lines. In this study, two highly conserved serine-threonine protein kinases, sgk-1 and sgk-2, were cloned from rectal gland tissue of the spiny dogfish (Squalus acanthias). Both kinases showed a distinct pattern of tissue specificity, with high expression levels in kidney, intestine, liver and heart. In rectal gland slices sgk-1 transcription was induced by exposure to hypertonic solution, reduction of the extracellular urea concentration, and addition of the secretagogues vasoactive intestinal polypeptide (VIP) and carbachol. The shark sgk-1 serine-threonine protein kinase may therefore provide a link between cell volume, Cl–secretion and protein phosphorylation state in shark rectal gland cells.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 21 January 1998 / Received after revision: 8 April 1998 / Accepted: 28 April 1998
Rights and permissions
About this article
Cite this article
Waldegger, S., Barth, P., Forrest Jr., J. et al. Cloning of sgk serine-threonine protein kinase from shark rectal gland – a gene induced by hypertonicity and secretagogues. Pflügers Arch 436, 575–580 (1998). https://doi.org/10.1007/s004240050674
Issue Date:
DOI: https://doi.org/10.1007/s004240050674