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The diageotropica mutation of tomato disrupts a signalling chain using extracellular auxin binding protein 1 as a receptor

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Abstract

The diageotropica (dgt) mutant of tomato (Lycopersicon esculentum Mill.) is known to lack a number of typical auxin responses. Here we show that rapid auxin-induced growth of seedling hypocotyls is completely abolished by the mutation over the full range of auxin concentrations tested, and also in early phases of the time course. Protoplasts isolated from wild-type hypocotyls respond to auxin by a rapid increase in cell volume, which we measured by image analysis at a high temporal resolution. A similar swelling could be triggered by antibodies directed against a part of the putative auxin-binding domain (box-a) of the auxin-binding protein 1 (ABP1). Induction of swelling both by auxin and by the antibody was not observed in the protoplasts isolated from the dgt mutant. However, dgt protoplasts are able to respond to the stimulator of the H+-ATPase, fusicoccin, with normal swelling. We propose that dgt is a signal-transduction mutation interfering with an auxin-signalling pathway that uses ABP1 as a receptor.

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Fig. 1a–f.
Fig. 2a, b.
Fig. 3.
Fig. 4. a

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Abbreviations

ABP:

auxin-binding protein

CCD:

charge-coupled device

2,4-D:

2,4-dichlorophenoxyacetic acid

dgt :

diageotropica

FC:

fusicoccin

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Acknowledgements

We express our thanks to Dr. Catharina Coenen for giving a generous supply of dgt and wild-type seeds. Dr. Christian Feckler and Prof. Klaus Palme (Universität Freiburg) supplied the anti-intern antibodies used in this study. We also thank the Deutsche Forschungsgemeinschaft for funding within the program "molecular analysis of phytohormone action". This work is based in part on the PhD thesis of May Christian at the University of Hamburg.

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Authors and Affiliations

  1. Institut für Allgemeine Botanik der Universität, Ohnhorststr. 18, 22609, Hamburg, Germany

    May Christian, Daniel Schenck & Hartwig Lüthen

  2. Botanisches Institut und Botanischer Garten der Christian-Albrechts-Universität zu Kiel, Olshausenstr. 40, 24098, Kiel, Germany

    Bianka Steffens

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  1. May Christian
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  2. Bianka Steffens
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  3. Daniel Schenck
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  4. Hartwig Lüthen
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Correspondence to Hartwig Lüthen.

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Christian, M., Steffens, B., Schenck, D. et al. The diageotropica mutation of tomato disrupts a signalling chain using extracellular auxin binding protein 1 as a receptor. Planta 218, 309–314 (2003). https://doi.org/10.1007/s00425-003-1090-8

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  • DOI: https://doi.org/10.1007/s00425-003-1090-8

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