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Expression of KS-type dehydrins is primarily regulated by factors related to organ type and leaf developmental stage during vegetative growth

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Abstract

The expression of a gene, designated as DHN10, was analyzed at the protein level in two Solanum species. The DHN10 protein displays some consensus amino acid sequences of dehydrins, termed K- and S-segments. Unlike most dehydrins, both segments occur only in single copies in the DHN10 sequence and the S-segment is at a C-terminal position. Database searches revealed that KS-type dehydrins constitute a specific subclass distributed in dicotyledons and monocotyledons. In Solanum tuberosum L. plants, a high DHN10 abundance was observed under control conditions, particularly in flowers, stems, tubers and young developing leaves. In other Solanaceae and in barley (Hordeum vulgare L.), the amount of DHN10 was much more elevated in young leaves than in old leaves. DHN10 abundance was investigated in two Solanum species subjected to low temperature or to drought. Under stress conditions, we observed substantially higher protein levels only in mature expanded leaves. These findings clearly indicate that KS-type dehydrins are present at a high level in the absence of stress during vegetative growth and that their expression is primarily regulated by factors related to organ type and to leaf development stage. A potential role for the DHN10 dehydrin during plant development and in tolerance to environmental stress is discussed.

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Abbreviations

DHN10 :

Dehydrin protein of 10 kDa

His:

Histidine

KS-type dehydrin :

Dehydrin containing a single K-segment followed by a single S-segment

LEA:

Late embryogenesis abundant

NTS:

Nuclear targeting signal

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Acknowledgements

The authors thank Barbara Wójtowicz and Françoise Eymery for helpful assistance in growing plant material, and the GRAP (Groupe de Recherches Appliquées en Phytotechnologie) team for technical assistance in low-temperature experiments. This work was supported in part by the State Committee for Scientific Research, grant No. 6 PO6A 038 20.

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Authors and Affiliations

  1. Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland

    T. Rorat, W. J. Grygorowicz & W. Irzykowski

  2. Direction des Sciences du Vivant, Département d’Ecophysiologie Végétale et Microbiologie, Laboratoire d’Ecophysiologie de la Photosynthèse, CEA/Cadarache, 13108, Saint-Paul-lez-Durance, Cedex, France

    P. Rey

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  1. T. Rorat
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Correspondence to T. Rorat.

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Rorat, T., Grygorowicz, W.J., Irzykowski, W. et al. Expression of KS-type dehydrins is primarily regulated by factors related to organ type and leaf developmental stage during vegetative growth. Planta 218, 878–885 (2004). https://doi.org/10.1007/s00425-003-1171-8

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  • DOI: https://doi.org/10.1007/s00425-003-1171-8

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