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Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development

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Abstract

The transition from a green, hard, and acidic pericarp to a sweet, soft, coloured, and sugar-rich ripe fruit occurs in many unrelated fruit species. High throughput identification of differentially expressed genes in grape berry has been achieved by the use of 50-mers oligoarrays bearing a set of 3,200 Unigenes from Vitis vinifera to compare berry transcriptome at nine developmental stages. Analysis of transcript profiles revealed that most activations were triggered simultaneously with softening, occurring within only 24 h for an individual berry, just before any change in colouration or water, sugar, and acid content can be detected. Although most dramatically induced genes belong to unknown functional categories, numerous changes occur in the expression of isogenes involved in primary and secondary metabolism during ripening. Focusing on isogenes potentially significant in development regulation (hormonal control of transcription factor) revealed a possible role for several hormones (cytokinin, gibberellin, or jasmonic acid). Transcription factor analysis revealed the induction of RAP2 and WRKY genes at véraison, suggesting increasing biotic and abiotic stress conditions during ripening. This observation was strengthened by an increased expression of multiple transcripts involved in sugar metabolism and also described as induced in other plant organs during stress conditions. This approach permitted the identification of new isogenes as possible control points: a glutathione S-transferase exhibits the same expression profile as anthocyanin accumulation and a new putative sugar transporter is induced in parallel with sugar import.

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Abbreviations

EST:

Expressed sequence tag

GRIP:

Grape ripening induced protein

UGFT:

UDP glucose-flavonoid 3-O-glucosyl transferase

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Acknowledgements

This research was supported by a Genoplant grant n° CI 2001003 entitled “Grapevine genomics: Grape berry development and ripening, transcriptomic analysis and identification of interest genes”.

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  1. Unité Mixte de Recherche S.P.O., Biologie Intégrative de la Vigne et du Raisin, I.N.R.A., 2 Place Viala, 34060, Montpellier Cedex 1, France

    Nancy Terrier, Jérôme Grimplet, Philippe Abbal, Agnès Ageorges & Charles Romieu

  2. Unité Mixte de Recherche C.N.R.S. 6161, Transport des Assimilats, Université de Poitiers, Laboratoire de Physiologie, Biochimie et Biologie Moléculaire Végétales, 40 Avenue du Recteur Pineau, 86022, Poitiers, France

    David Glissant, Rossitza Atanassova, Fabienne Dédaldéchamp & Serge Delrot

  3. UMR 619, Equipe Biologie de la Vigne, Université de Bordeaux 1, 71 Avenue Edouard Bourleaux, 33883, Villenave d’Ornon Cedex, France

    François Barrieu, Carole Couture, Céline Léon, Jean-Pierre Renaudin & Saïd Hamdi

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  1. Nancy Terrier
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  2. David Glissant
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Correspondence to Charles Romieu.

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Terrier, N., Glissant, D., Grimplet, J. et al. Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development. Planta 222, 832–847 (2005). https://doi.org/10.1007/s00425-005-0017-y

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