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Influence of the photoperiod on redox regulation and stress responses in Arabidopsis thaliana L. (Heynh.) plants under long- and short-day conditions

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Abstract

Arabidopsis thaliana L. (Heynh.) plants were grown in low light (150 μmol photons m−2 s−1 and 20°C) either in short days (7.5 h photoperiod) or long days (16 h photoperiod), and then transferred into high light and low temperature (350–800 μmol photons m−2 s−1 at 12°C). Plants grown in short days responded with a rapid increase in NADP-malate dehydrogenase (EC 1.1.1.82) activation state. However, persisting overreduction revealed a new level of regulation of the malate valve. Activity measurements and Northern-blot analyses indicated that NADP-malate dehydrogenase transcript and protein levels increased within a few hours. Using macroarrays, additional changes in gene expression were identified. Transcript levels for several enzymes of glutathione metabolism and of some photosynthetic genes increased. The cellular glutathione level increased, but its redox state remained unchanged. A different situation was observed in plants grown in long-day conditions. Neither NADP-malate dehydrogenase nor glutathione content changed, but the expression of several antioxidative enzymes increased strongly. We conclude that the endogenous systems that measure day length interact with redox regulation, and override the interpretation of the signals, i.e. they redirect redox-mediated acclimation signals to allow for more efficient light usage and redox poising in short days to systems for the prevention of oxidative damages when grown under long-day conditions.

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Abbreviations

Apx:

Ascorbate peroxidase

Cat:

Catalase

DTT:

Dithiothreitol

GR:

Glutathione reductase

GS:

Glutathione synthase

LD:

Long day

LHC:

Light-harvesting complex

MDHAR:

Monodehydroascorbate reductase

MDH:

Malate dehydrogenase

NTR:

NADP-thioredoxin reductase

SD:

Short day

SOD:

Superoxide dismutase

Trx:

Thioredoxin

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Acknowledgements

The authors thank H. Rennenberg and M. Eiblmeyer (Universitaet Freiburg, Germany) for their help with the method of glutathione determination. Further thanks are due to S. Klocke for her help with performing the experiments, and R. Brockmann for the frustrating job of measuring the NADP-MDH activities in Arabidopsis plants grown in mixed SD and LD conditions. We finally thank H. Wolf-Wibbelmann and S. Steinbach for excellently growing the plant material. This work was financially supported by a grant from the Deutsche Forschungsgemeinschaft (FOR 387, TP1 and TP3).

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Authors and Affiliations

  1. Pflanzenphysiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, 49069, Osnabrück, Germany

    Beril Becker, Simone Holtgrefe, Sabrina Jung, Christina Wunrau, Jan E. Backhausen & Renate Scheibe

  2. Lehrstuhl für Biochemie und Physiologie der Pflanzen, Fakultät für Biologie, Universität Bielefeld, 10 01 31, 33501, Bielefeld, Germany

    Andrea Kandlbinder, Margarete Baier & Karl-Josef Dietz

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  1. Beril Becker
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  2. Simone Holtgrefe
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  3. Sabrina Jung
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Correspondence to Jan E. Backhausen.

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Becker, B., Holtgrefe, S., Jung, S. et al. Influence of the photoperiod on redox regulation and stress responses in Arabidopsis thaliana L. (Heynh.) plants under long- and short-day conditions. Planta 224, 380–393 (2006). https://doi.org/10.1007/s00425-006-0222-3

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