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Similar mechanisms might be triggered by alternative external stimuli that induce dormancy release in grape buds

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Abstract

The detection of genes having similar expression profiles following the application of different stimuli that trigger bud break may constitute potent tools for the identification of pathways with a central role in dormancy release. We compared the effects of heat shock (HS) and hydrogen cyanamide (HC) and demonstrated that HS leads to earlier and higher bud-break levels. Changes in transcript levels of catalase, alcohol dehydrogenase and pyruvate decarboxylase were induced following both treatments. However, timing and extent of changes in transcript level differed. Changes occurred earlier in HS-treated buds and were more intense in HC-treated buds. The changes in transcript levels after both treatments were temporary. The rapid and short-lasting changes in gene expression following HS treatment correlated with the faster and higher level of bud-break that this treatment exerted. This correlation may propose that the reported molecular events are mechanistically involved in dormancy release. To test the hypothesis that temporary oxidative stress is part of the mechanism inducing dormancy release, we analyzed the effect of HS and HC treatments on the expression of ascorbate peroxidase, glutathione reductase, thioredoxin h, glutathione S-transferase and sucrose synthase genes and found that they were induced by both treatments in a similar pattern. Taken together, these findings propose that similar cellular processes might be triggered by different stimuli that lead to dormancy release, and are consistent with the hypothesis that temporary oxidative stress and respiratory stress might be part of the mechanism that leads to bud break.

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Abbreviations

ADH:

Alcohol dehydrogenase

APX:

Ascorbate peroxidase

CAT:

Catalase

CDPK:

Calcium-dependent protein kinase

EST:

Expressed sequence tag

GR:

Glutathione reductase

GST:

Glutathione S-transferase

HC:

Hydrogen cyanamide

HS:

Heat shock

PDC:

Pyruvate decarboxylase

RE:

Relative expression

ROS:

Reactive oxygen species

SDS:

Sodium dodecyl sulfate

StSy:

Stilben synthase

SuSy:

Sucrose synthase

TrH:

Thioredoxin h

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Acknowledgments

This research was supported by Research Grant Award No. IS-3340-02 from BARD, The United States-Israel Binational Agricultural Research and Development Fund.

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Author notes

  1. Xuequn Pang

    Present address: College of Life Sciences, South China Agricultural University, 510642, Guangzhou, China

  2. Jaganatha Venkateswari

    Present address: Department of Botany and Plant Sciences, University of California, 2150 BatchelorHall, Riverside, CA, 92521, USA

Authors and Affiliations

  1. Department of Fruit Tree Sciences, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, P.O. Box 6, 50250, Bet Dagan, Israel

    Tamar Halaly, Xuequn Pang, Tamar Batikoff, Omer Crane, Alexandra Keren, Jaganatha Venkateswari, Aliza Ogrodovitch, Avi Sadka, Shimon Lavee & Etti Or

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  1. Tamar Halaly
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Correspondence to Etti Or.

Additional information

T. Halaly and X. Pang contributed equally to the paper.

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Halaly, T., Pang, X., Batikoff, T. et al. Similar mechanisms might be triggered by alternative external stimuli that induce dormancy release in grape buds. Planta 228, 79–88 (2008). https://doi.org/10.1007/s00425-008-0720-6

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