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Expression analysis of the auxin efflux carrier family in tomato fruit development

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Abstract

Auxin transport network, which is important in the integration of plant developmental signals, depends on differential expression of the auxin efflux carrier PIN gene family. We cloned three tomato PIN (referred as SlPIN) cDNAs and examined their expression patterns in fruit and other organs. The expression of SlPIN1 and SlPIN2 was highest in very young fruit immediately after anthesis, whereas the expression of SlPIN3 was low at this same stage of fruit development. SlPIN2::GUS was expressed in ovules at anthesis and in young developing seeds at 4 days after anthesis, while SlPIN1::GUS was expressed in whole fruit. The DR5::GUS auxin-responsive reporter gene was expressed in the fruit and peduncle at anthesis and was higher in the peduncle 4 days after anthesis. These studies suggest that auxin is likely transported from young seeds by SlPIN1 and SlPIN2 and accumulated in peduncles where SlPIN gene expression is low in tomato. The possible role of SlPINs in fruit set was discussed.

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Abbreviations

GUS:

β-Glucuronidase

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Author notes

  1. Sogo Nishio

    Present address: National Institute of Fruit Tree Science, Tsukuba, 305-8605, Japan

Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan

    Sogo Nishio, Ryo Moriguchi, Hiroki Ikeda, Hideki Takahashi, Koki Kanahama & Yoshinori Kanayama

  2. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8578, Japan

    Hideyuki Takahashi & Nobuharu Fujii

  3. Department of Biochemistry, University of Missouri, Columbia, Columbia, MO, 65211, USA

    Thomas J. Guilfoyle

Authors
  1. Sogo Nishio
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  2. Ryo Moriguchi
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  3. Hiroki Ikeda
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  4. Hideki Takahashi
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  5. Hideyuki Takahashi
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  6. Nobuharu Fujii
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  7. Thomas J. Guilfoyle
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  9. Yoshinori Kanayama
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Correspondence to Yoshinori Kanayama.

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Nishio, S., Moriguchi, R., Ikeda, H. et al. Expression analysis of the auxin efflux carrier family in tomato fruit development. Planta 232, 755–764 (2010). https://doi.org/10.1007/s00425-010-1211-0

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  • DOI: https://doi.org/10.1007/s00425-010-1211-0

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