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Wheat vacuolar H+-ATPase subunit B cloning and its involvement in salt tolerance

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Abstract

Wheat vacuolar H+-ATPases (V-ATPase) subunit B, named TaVB, was isolated from the salt-tolerant wheat RH8706-49 and used to transform Arabidopsis plants. TaVB-expressed Arabidopsis has a higher germination rate, root length, V–H+-ATPase activity, and overall salt tolerance than the wild type, indicating that expression of the gene can affect salt tolerance of the transgenic plants. Under salt stress, intracellular Na+ levels in transgenic plants are significantly lower than the control. These results suggest that expression of the wheat TaVB gene may enhance plant tolerance to salt stress.

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Abbreviations

V–H+-ATPase:

Vacuolar H+-ATPase

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Acknowledgments

This work was supported by National Natural Science Fund (30971766) and Hebei Provincial Natural Science Fund (No. C2009000278).

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Authors and Affiliations

  1. College of Life Science of Hebei Normal University, No. 113 Yuhua East Road, Shijiazhuang, Hebei, China

    Li Wang, Xiaoliang He, Yanjun Zhao, Yinzhu Shen & Zhanjing Huang

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  1. Li Wang
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  2. Xiaoliang He
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  3. Yanjun Zhao
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  4. Yinzhu Shen
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  5. Zhanjing Huang
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Corresponding author

Correspondence to Zhanjing Huang.

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L. Wang and X. He are co-first authors.

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Wang, L., He, X., Zhao, Y. et al. Wheat vacuolar H+-ATPase subunit B cloning and its involvement in salt tolerance. Planta 234, 1–7 (2011). https://doi.org/10.1007/s00425-011-1383-2

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