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Quantitative genetic analysis of salicylic acid perception in Arabidopsis

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Abstract

Salicylic acid (SA) is a phytohormone required for a full resistance against some pathogens in Arabidopsis, and NPR1 (Non-Expressor of Pathogenesis Related Genes 1) is the only gene with a strong effect on resistance induced by SA which has been described. There can be additional components of SA perception that escape the traditional approach of mutagenesis. An alternative to that approach is searching in the natural variation of Arabidopsis. Different methods of analyzing the variation between ecotypes have been tried and it has been found that measuring the growth of a virulent isolate of Pseudomonas syringae after the exogenous application of SA is the most effective one. Two ecotypes, Edi-0 and Stw-0, have been crossed, and their F2 has been studied. There are two significant quantitative trait loci (QTLs) in this population, and there is one QTL in each one of the existing mapping populations Col-4 × Laer-0 and Laer-0 × No-0. They have different characteristics: while one QTL is only detectable at low concentrations of SA, the other acts after the point of crosstalk with methyl jasmonate signalling. Three of the QTLs have candidates described in SA perception as NPR1, its interactors, and a calmodulin binding protein.

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Abbreviations

BTH:

Benzothiadiazole

MeJA:

Methyl jasmonate

NIL:

Near isogenic lines

NPR1 :

Non-Expressor of Pathogenesis Related Genes 1

QTL:

Quantitative trait loci

RIL:

Recombinant inbreed lines

SA:

Salicylic acid

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Acknowledgments

This work was supported by the Ministerio de Ciencia e Innovación (MICINN) of Spain (grant BIO201018896 to PT, a JAE-CSIC Fellowship to JVC and a FPI-MICINN to AD). We appreciate the BTH provided by Syngenta and the genotyping by CEGEN (Fundación Genoma España).

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The authors declare that they have no conflict of interest.

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Correspondence to Pablo Tornero.

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A. Dobón and J. V. Canet contributed equally to this work.

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Dobón, A., Canet, J.V., Perales, L. et al. Quantitative genetic analysis of salicylic acid perception in Arabidopsis. Planta 234, 671–684 (2011). https://doi.org/10.1007/s00425-011-1436-6

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