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Automated in situ detection (AISD) of biomolecules

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Abstract

 In order to facilitate in situ detection of biomolecules in large sample series the processing of whole-mount specimens has been automated. A freely programmable liquid handling system is described by which embryos or similar biological materials are processed. Possible applications include in situ hybridization (ISH), immunocytochemistry (ICC) or reporter gene assays. Process times required for the preparation of whole-mount in situ hybridizations in Drosophila, Xenopus, Gallus and in hydroids were – in part – significantly reduced as compared with manual processing. Application of automated in situ detection (AISD) in random screening is demonstrated in hydroids. Potential further applications are discussed.

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Authors and Affiliations

  1. Zoologisches Institut, Universität zu Köln, Weyertal 119, D-50923 Köln, Germany, , , , , , DE

    G. Plickert, Martin Gajewski, Gerd Gehrke, Jörg Schlossherr & Hady Ibrahim

  2. Abimed-Analysentechnik, Raiffeisenstrasse 3, D-40764 Langenfeld, Germany, , , , , , DE

    Heinrich Gausepohl

Authors
  1. G. Plickert
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  2. Martin Gajewski
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  3. Gerd Gehrke
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  4. Heinrich Gausepohl
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  5. Jörg Schlossherr
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  6. Hady Ibrahim
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Additional information

Received: 13 May 1997 / Accepted: 27 June 1997

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Plickert, G., Gajewski, M., Gehrke, G. et al. Automated in situ detection (AISD) of biomolecules. Dev Gene Evol 207, 362–367 (1997). https://doi.org/10.1007/s004270050124

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  • DOI: https://doi.org/10.1007/s004270050124

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