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Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins

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Abstract

 We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 12/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.

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Authors and Affiliations

  1. Brookdale Center for Molecular Biology, Box 1126, Mt. Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA, , , , , , US

    David Kosman & J. Reinitz

  2. Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA, , , , , , US

    Stephen Small

Authors
  1. David Kosman
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  2. Stephen Small
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  3. J. Reinitz
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Received: 2 February 1998 / Accepted: 19 February 1998

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Kosman, D., Small, S. & Reinitz, J. Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins. Dev Gene Evol 208, 290–294 (1998). https://doi.org/10.1007/s004270050184

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  • DOI: https://doi.org/10.1007/s004270050184

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