Mixed mucinous-type and non-mucinous-type adenocarcinoma of the lung: immunohistochemical examination and K-ras gene mutation

Abstract.

Mucinous-type adenocarcinoma and non-mucinous-type adenocarcinoma are known to be the representative histological subtypes of bronchioloalveolar carcinoma. Mucinous-type adenocarcinoma is also known to carry abnormalities of the K-ras gene at high frequency. However, the mixed subtype of the both mucinous-type and non-mucinous-type adenocarcinoma (mixed-type) has not been analyzed in detail, although its existence has been reported in a few papers. In this study we carried out immunohistochemical and molecular biological analyses of 15 examples of the mixed-type, in comparison with 11 cases of mucinous-type and 21 cases of non-mucinous-type adenocarcinoma. Immunohistochemically, lysozyme – one of the specific markers of mucinous-type adenocarcinoma – was not stained in the mucinous component of the mixed-type. K-ras gene mutations were detected only in mucinous-type (73%) and non-mucinous-type (10%) adenocarcinomas and not in either the mucinous or non-mucinous component of the mixed-type (0%). Therefore, although mixed-type adenocarcinomas consist of tumor cells showing both mucinous and non-mucinous morphology, the mucinous component of this type differs from mucinous-type adenocarcinoma in terms of immunohistochemical features and K-ras gene alteration.