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Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction

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Abstract

Recent evidence suggests that oxidative stress is involved in the pathophysiology of preeclampsia. Using immunohistochemistry and Western blotting, we investigated the oxidative stress- and redox-related molecules, such as 8-hydroxy-2′-deoxyguanosine (8-OHdG), 4-hydroxynonenal (4-HNE), thioredoxin (TRX) and redox factor-1 (ref-1) in the placenta in preeclampsia, intrauterine growth restriction (IUGR), preeclampsia + IUGR and in normal pregnancy. Using immunohistochemistry, the level of 8-OHdG was significantly higher in IUGR (P=0.012) or preeclampsia + IUGR (P=0.0021) than in normal pregnancy, while TRX expression was significantly higher in preeclampsia (P=0.045), and ref-1 expression was significantly higher in preeclampsia (P=0.017), IUGR (P=0.016) and preeclampsia + IUGR (P=0.0038) than in normal pregnancy. The levels of 4-HNE did not differ significantly between either preeclampsia or IUGR and normal pregnancy. A significant positive correlation was observed between TRX and ref-1 expressions in both normal (ρ=0.52) and complicated (ρ=0.43) pregnancies. Using Western blotting, ref-1 expression tended to be higher in complicated pregnancies than in normal pregnancy (P=0.09). These results suggest that oxidative DNA damage is increased in IUGR and that redox function is enhanced in both preeclampsia and IUGR compared with normal pregnancy.

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Acknowledgement

The authors thank Professor Junji Yodoi, Kyoto University, for providing the antibody against thioredoxin.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Shinshu University School of Medicine, Matsumoto, Japan

    Yasushi Takagi, Toshihiko Toki, Naoko Kita, Makoto Kanai, Takashi Ashida, Satoshi Ohira & Ikuo Konishi

  2. Department of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, 390-8621, Matsumoto , Japan

    Toshio Nikaido

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  1. Yasushi Takagi
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  2. Toshio Nikaido
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  3. Toshihiko Toki
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Correspondence to Toshio Nikaido.

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Takagi, Y., Nikaido, T., Toki, T. et al. Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction. Virchows Arch 444, 49–55 (2004). https://doi.org/10.1007/s00428-003-0903-2

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  • DOI: https://doi.org/10.1007/s00428-003-0903-2

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