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L-methionine-induced alterations in molecular signatures in MCF-7 and LNCaP cancer cells

  • Original Paper
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Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Background

Methionine inhibits proliferation of breast and prostate cancer cells. Here, we determined the influence of L-methionine on functional molecular signatures in these cell lines.

Methods

MCF-7 and LNCaP cells were treated with L-methionine (5 mg/ml) for 72 h. Changes in molecular signatures of these cells were examined by microarray analysis of 15,814 probes in triplicate experiments.

Results

In LNCaP cells, 325 genes were up-regulated by methionine, and 517 genes down-regulated. In MCF-7 cells, 86 genes were up-regulated and 135 genes down-regulated. Ninety-eight genes were regulated in the same direction by methionine in both cells lines, and five other genes were changed in expression in opposite directions.

Conclusion

Several of the up-regulated genes encode proteins involved in cellular redox regulation, suggesting that methionine may enhance antioxidant mechanisms. Many of the down-regulated genes belong to protein kinase families that may be related to the anti-proliferative effects of methionine on breast and prostate cancer cells.

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Acknowledgments

Supported in part by a Supplement to NIH Grant No. R01CA116195. The authors thank Dr Jin-Qiang Chen and Dr Daniel Guimaraes Tiezzi for technical and edition assistance.

Conflict of interest statement

We declare that we have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Pathology, College of Medicine, University of Illinois at Chicago, 840 South Wood Street, Room 130 CSN, MC 847, Chicago, IL, 60612, USA

    Maximo A. Benavides, Dong Hu, Wancai Yang & Maarten C. Bosland

  2. DNA Service Laboratory, Research Resources Center, University of Illinois at Chicago, Chicago, IL, USA

    Marie Kristine Baraoidan & Annette Bruno

  3. Biomedical Informatics Center, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA

    Pan Du & Simon Lin

  4. Department of Surgery, University of Alabama at Birmingham School of Medicine, Birmingham, AL, USA

    Kirby I. Bland

  5. Department of Pathology, University of Alabama at Birmingham School of Medicine, Birmingham, AL, USA

    William E. Grizzle

Authors
  1. Maximo A. Benavides
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  2. Dong Hu
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  3. Marie Kristine Baraoidan
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  4. Annette Bruno
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  5. Pan Du
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  6. Simon Lin
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  7. Wancai Yang
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  8. Kirby I. Bland
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  9. William E. Grizzle
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  10. Maarten C. Bosland
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Corresponding authors

Correspondence to Maximo A. Benavides or Maarten C. Bosland.

Electronic supplementary material

Below is the link to the electronic supplementary material.

432_2010_897_MOESM1_ESM.pdf

Supplemental Table 1: Genes with altered expression in LNCaP cells treated with L-methionine 5 mg/ml for 72 h. Significant changes in expression were observed in a total of 842 genes out of 15,814 probes examined, with 325 genes up-regulated and 517 genes down-regulated. (PDF 208 kb)

432_2010_897_MOESM2_ESM.pdf

Supplemental Table 2: Genes with altered expression in MCF-7 cells treated with L-methionine 5 mg/ml for 72 h. Significant changes in expression were observed in a total of 221 genes out of 15,814 probes examined, with 86 genes were up-regulated and 135 genes down-regulated. (PDF 80 kb)

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Benavides, M.A., Hu, D., Baraoidan, M.K. et al. L-methionine-induced alterations in molecular signatures in MCF-7 and LNCaP cancer cells. J Cancer Res Clin Oncol 137, 441–453 (2011). https://doi.org/10.1007/s00432-010-0897-5

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  • DOI: https://doi.org/10.1007/s00432-010-0897-5

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