Abstract
As Trypanosoma cruzi, the etiological agent of Chagas disease, multiplies in the cytoplasm of nucleated host cells, infection with this parasite is highly likely to affect host cells. We performed an exhaustive transcriptome analysis of T. cruzi-infected HeLa cells using an oligonucleotide microarray containing probes for greater than 47,000 human gene transcripts. In comparison with uninfected cells, those infected with T. cruzi showed greater than threefold up-regulation of 41 genes and greater than threefold down-regulation of 23 genes. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) of selected, differentially expressed genes confirmed the microarray data. Many of these up- and down-regulated genes were related to cellular proliferation, including seven up-regulated genes encoding proliferation inhibitors and three down-regulated genes encoding proliferation promoters, strongly suggesting that T. cruzi infection inhibits host cell proliferation, which may allow more time for T. cruzi to replicate and produce its intracellular nests. These findings provide new insight into the molecular mechanisms by which intracellular T. cruzi infection influences the host cell, leading to pathogenicity.
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Acknowledgement
This work was supported in part by Grants-in-Aid (Nos. 17390123 and 18790297) for Scientific Research from the Ministry of Education, Sports, Science, Culture, and Technology (ESSCT) of Japan. This study was in part supported by the High Technology Research Center Grant from Ministry of ESSCT of Japan. MH and TA are supported by 21st Century COE Research from the Ministry of ESSCT of Japan. MH is supported by a Grant-in-Aid for 21st Century COE Research.
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Shigihara, T., Hashimoto, M., Shindo, N. et al. Transcriptome profile of Trypanosoma cruzi-infected cells: simultaneous up- and down-regulation of proliferation inhibitors and promoters. Parasitol Res 102, 715–722 (2008). https://doi.org/10.1007/s00436-007-0819-x
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DOI: https://doi.org/10.1007/s00436-007-0819-x