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Structural and functional analysis of 5S rRNA in Saccharomyces cerevisiae

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Abstract

5S rRNA extends from the central protuberance of the large ribosomal subunit, through the A-site finger, and down to the GTPase-associated center. Here, we present a structure-function analysis of seven 5S rRNA alleles which are sufficient for viability in the yeast Saccharomyces cerevisiae when expressed in the absence of wild-type 5S rRNAs, and extend this analysis using a large bank of mutant alleles that show semi-dominant phenotypes in the presence of wild-type 5S rRNA. This analysis supports the hypothesis that 5S rRNA serves to link together several different functional centers of the ribosome. Data are also presented which suggest that in eukaryotic genomes selection has favored the maintenance of multiple alleles of 5S rRNA, and that these may provide cells with a mechanism to post-transcriptionally regulate gene expression.

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Acknowledgements

We wish to thank the members of the Dinman and Dontsova laboratories, with special thanks to Sarah Fraser, Ewan Plant, Steve Hutcheson and Alexey Bogdanov for their advice and support. This work was supported by grants to JDD from the National Institutes of Health (GM62143), and to JDD and OAD from the Fogarty International Center (TW005787), and to OAD from HHMI 55000303 and RFBR.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Building A, Room 608, Vorobievy Gory, Moscow, 119899, Russia

    Sergey Kiparisov, Petr V. Sergiev & Olga A. Dontsova

  2. Department of Cell Biology and Molecular Genetics, University of Maryland, Microbiology Building Room 2135, College Park, MD, 20742, USA

    Alexey Petrov, Arturas Meskauskas & Jonathan D. Dinman

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  1. Sergey Kiparisov
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  2. Alexey Petrov
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  3. Arturas Meskauskas
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  4. Petr V. Sergiev
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  5. Olga A. Dontsova
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  6. Jonathan D. Dinman
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Corresponding author

Correspondence to Jonathan D. Dinman.

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Sergey Kiparisov and Alexey Petrov contributed equally to this work

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Kiparisov, S., Petrov, A., Meskauskas, A. et al. Structural and functional analysis of 5S rRNA in Saccharomyces cerevisiae . Mol Genet Genomics 274, 235–247 (2005). https://doi.org/10.1007/s00438-005-0020-9

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