Abstract
The accumulation of Ade+ revertants during adenine starvation and Trp+ revertants during tryptophan starvation in haploid polyauxotrophic strains of Saccharomyces cerevisiae occurs in a time-dependent manner. Accumulation of revertants is enhanced in Rad6− strains, suggesting that starvation-induced reversion is influenced by some of the RAD6 gene functions. The higher frequency of adaptive reversions in Rad6− strains is somewhat influenced by, but does not totally depend on, the genetic background. Therefore, the RAD6 gene product is involved in maintaining a low level not only of spontaneous mutation but also of starvation-induced reversion. The starvation-induced Ade+ and Trp+ reversions both appear to be adaptive. The analysis of growth characteristics and the genotype of revertants shows a difference between early and late-appearing revertants. These results support the hypothesis that the adaptivity of starvation-induced reversion is based on the selective fixation of random mutations, and particularly on transcription-enhanced repair and/or mutagenesis processes.
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Received: 2 October 1997 / Accepted: 20 March 1998
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Storchová, Z., Rojas Gil, A., Janderová, B. et al. The involvement of the RAD6 gene in starvation-induced reverse mutation in Saccharomyces cerevisiae . Mol Gen Genet 258, 546–552 (1998). https://doi.org/10.1007/s004380050766
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DOI: https://doi.org/10.1007/s004380050766