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Influence of dissolved oxygen and shear conditions on clavulanic acid production by Streptomyces clavuligerus

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Abstract

Clavulanic acid (CA), a potent β-lactamase inhibitor, is produced by a filamentous bacterium. Here, the effect of DO and shear, expressed as impeller tip velocity, on CA production was examined. Cultivations were performed in a 4 L fermentor with speeds of 600, 800 and 1,000 rpm and a fixed air flow rate (0.5 vvm). Also, cultivation with automatic control of dissolved oxygen, at 50% air saturation, by varying stirrer speed and using a mixture of air and O2 (10% v/v) in the inlet gas, and a cultivation with fixed stirrer speed of 800 rpm and air flow rate of 0.5 vvm, enriched with 10% v/v O2, were performed. Significant variations in CA titer, CA production rate and O2 uptake-rate were observed. It was also found that the DO level has no remarkable effect on CA production once a critical level is surpassed. The most significant improvement in CA production was related to high stirrer speeds.

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Acknowledgements

This work was supported by FAPESP—Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil (Grant Proc. 00/01383-0 and Grant Proc. 03/11722-5).

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  1. Department of Chemical Engineering, Universidade Federal de São Carlos, Cx. Postal 676, CEP 13565-905, São Carlos SP, Brazil

    J. C. Rosa, A. Baptista Neto, C. O. Hokka & A. C. Badino

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  1. J. C. Rosa
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  2. A. Baptista Neto
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  3. C. O. Hokka
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  4. A. C. Badino
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Correspondence to A. C. Badino.

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Rosa, J.C., Neto, A.B., Hokka, C.O. et al. Influence of dissolved oxygen and shear conditions on clavulanic acid production by Streptomyces clavuligerus . Bioprocess Biosyst Eng 27, 99–104 (2005). https://doi.org/10.1007/s00449-004-0386-9

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  • DOI: https://doi.org/10.1007/s00449-004-0386-9

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