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Hydrogen-rich saline solution attenuates renal ischemia–reperfusion injury

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Abstract

Purpose

Renal ischemia–reperfusion (I/R), an important cause of acute kidney injury, is unavoidable during various types of operations, including renal transplantation, surgical revascularization of the renal artery, partial nephrectomy, and treatment of suprarenal aortic aneurysms. Exacerbation of I/R injury is mediated by reactive oxygen species (ROS). A recent study has shown that hydrogen has antioxidant properties. In this study, we tested the hypothesis that a hydrogen-rich saline solution (HRSS) attenuates renal I/R injury in a rodent model.

Methods

Rats were treated with an intravenous injection of HRSS or control saline solution followed by renal I/R. After 24 h of treatment, we performed a histological examination and transmission electron microscopy, and measured serum levels of 8-OHdG.

Results

Histological analysis revealed a marked reduction of interstitial congestion, edema, inflammation, and hemorrhage in renal tissue harvested 24 h after HRSS treatment compared to saline administration. Renal I/R injury, which led to altered mitochondrial morphology, was also inhibited by HRSS. Furthermore, serum 8-OHdG levels were significantly lower in rats treated with HRSS and subjected to renal I/R.

Conclusions

These protective effects were likely due to the antioxidant properties of HRSS. These results suggest that HRSS is a potential therapeutic candidate for treating various I/R diseases.

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Acknowledgments

The authors thank Hiroaki Kawazato and Aiko Yasuda for their helpful advice on the preparation of kidney tissue specimens. We also thank Dr. Tomohisa Uchida for scoring renal histology. This study was supported by Grants-in-Aid for Scientific Research (Kakenhi).

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Authors and Affiliations

  1. Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasamamachi, Yufu, Oita, 879-5593, Japan

    Chihiro Shingu, Hironori Koga, Satoshi Hagiwara, Shigekiyo Matsumoto, Koji Goto & Takayuki Noguchi

  2. Department of Physiology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasamamachi, Yufu, Oita, 879-5593, Japan

    Isao Yokoi

Authors
  1. Chihiro Shingu
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  2. Hironori Koga
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  3. Satoshi Hagiwara
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  4. Shigekiyo Matsumoto
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  5. Koji Goto
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  6. Isao Yokoi
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  7. Takayuki Noguchi
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Corresponding author

Correspondence to Satoshi Hagiwara.

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Shingu, C., Koga, H., Hagiwara, S. et al. Hydrogen-rich saline solution attenuates renal ischemia–reperfusion injury. J Anesth 24, 569–574 (2010). https://doi.org/10.1007/s00540-010-0942-1

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  • DOI: https://doi.org/10.1007/s00540-010-0942-1

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