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Pro-inflammatory cytokines expression increases following low- and high-magnitude cyclic loading of lumbar ligaments

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Abstract

Repetitive or overuse disorders of the lumbar spine affect the lives of workers and athletes. We hypothesize that repetitive anterior lumbar flexion–extension under low or high load will result in significantly elevated pro-inflammatory cytokines expression several hours post-activity. High loads will exhibit significantly higher expression than low loads. Lumbar spine of in vivo feline was subjected to cyclic loading at 0.25 Hz for six 10-min periods with 10 min of rest in between. One group was subjected to a low peak load of 20 N, whereas the second group to a high peak load of 60 N. Following a 7-h post-loading rest, the supraspinous ligaments of L-3/4, L-4/5 and L-5/6 and the unstimulated T-10/11 were excised for mRNA analysis and IL-1β, IL-6, IL-8, TNFα and TGFβ1 pro-inflammatory cytokines expression. Creep (laxity) developed in the lumbar spine during the loading and the subsequent 7 h of rest was calculated. A two-way mixed model ANOVA was used to assess difference in each cytokines expression between the two groups and control. Tukey HSD post hoc analysis delineated specific significant effects. Significance was set at 0.05. Low and high-load groups exhibited development of creep throughout the cyclic loading period and gradual recovery throughout the 7-h rest period. Residual creep of 24.8 and 30.2% were present in the low and high-load groups, respectively, 7-h post-loading. Significant increases in expression of all cytokines measured relative to control were obtained for supraspinous ligaments from both low and high-load magnitudes. IL-6, IL-8 and TGFβ1 expression in the high-load group were significantly higher relative to the low-load group. Significant increases in cytokines expression indicating tissue inflammation are observed several hours post-repetitive lumbar flexion–extension regardless of the load magnitude applied. Repetitive occupational and athletic activity, regardless of the load applied, may be associated with the potential of developing acute inflammatory conditions that may convert to chronic inflammation if the viscoelastic tissues are further exposed to repetitive activity over long periods. Appropriate rest periods are a relevant preventive measure.

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Acknowledgments

This work was supported in part by grant RO1-OH-07622 from NIOSH and by the Academic Enrichment Fund from the UC Denver, School of Medicine. Peter D’Ambrosia, MD was a research Orthopedic resident supported by the Department of Orthopedics.

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  1. Musculoskeletal Disorders Research Laboratory, Bioengineering Division, Department of Orthopedic Surgery, Health Sciences Center, University of Colorado, Denver, 12800 E 19th Ave, RC-1N, Mail Stop 8343, Box 6511, Aurora, CO, 80045, USA

    Peter D’Ambrosia, Karen King, Bradley Davidson, Bing He Zhou, Yun Lu & Moshe Solomonow

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  1. Peter D’Ambrosia
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  3. Bradley Davidson
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Correspondence to Moshe Solomonow.

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D’Ambrosia, P., King, K., Davidson, B. et al. Pro-inflammatory cytokines expression increases following low- and high-magnitude cyclic loading of lumbar ligaments. Eur Spine J 19, 1330–1339 (2010). https://doi.org/10.1007/s00586-010-1371-4

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  • DOI: https://doi.org/10.1007/s00586-010-1371-4

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