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Mating system and genetic diversity in natural populations of Nelumbo nucifera (Nelumbonaceae) detected by ISSR markers

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Abstract

In this study, outcrossing rates and genetic diversity in natural populations of Nelumbo nucifera were investigated. The estimated multilocus outcrossing rate (tm) based on 28 ISSR loci was over 90%. Analysis of genetic diversity revealed that this index was high at the species level (Hs = 0.325, I = 0.514), but low within the individual study populations (Hs = 0.148, I = 0.212). Gst-B was 0.547 and Nm was 0.414. The results of AMOVA indicated that 54.6% of the variation was due to the difference between the regions and 45.4% to the variation within the region. Although the populations were predominantly outcrossing, most of the genetic diversity was attributed to geographical effects instead of their habitats because low sexual recruitment and clonal growth deeply reduced the genetic diversity within the populations. On the basis of the high tm, Gst-B and low Nm values, we recommended that any future conservation plans should include both in situ conservation and germplasm collection.

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Acknowledgments

We thank Prof. Wang QF and Dr. Chen JM (of the School of Life Sciences, Wuhan University), Prof. Ge S and Dr. Zhu QH (of the Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences) for their help with data analysis. This work was supported by the National Natural Science Foundation of China (No. 30771375 and No. 30471114) and National Science and Technology Pillar Program (No. 2007BAD37B06).

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Authors and Affiliations

  1. Key Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, 430072, Wuhan, China

    Yan-Chuang Han, Zhong-Li Hu & Yun-Chun Song

  2. College of Life Science, Guangxi Normal University, 541004, Guilin, China

    Cai-Zhu Teng

  3. Botany Department, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 63000-00200, Nairobi, Kenya

    Gituru Robert Wahiti

  4. Lotus Center, Wuhan University, 430072, Wuhan, China

    Ming-Quan Zhou

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  1. Yan-Chuang Han
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  2. Cai-Zhu Teng
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  3. Gituru Robert Wahiti
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  4. Ming-Quan Zhou
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  5. Zhong-Li Hu
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Correspondence to Ming-Quan Zhou or Zhong-Li Hu.

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Han, YC., Teng, CZ., Wahiti, G.R. et al. Mating system and genetic diversity in natural populations of Nelumbo nucifera (Nelumbonaceae) detected by ISSR markers. Plant Syst Evol 277, 13–20 (2009). https://doi.org/10.1007/s00606-008-0096-x

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