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Molecular diversity and phylogenetic analyses of y-type high-molecular-weight glutenin promoters from different genomes in Triticeae

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Abstract

Sequence polymorphisms and phylogenetic relationships from different genomes of 25 diploid species in Triticeae (Poaceae) were evaluated by using the sequences of y-type high-molecular-weight glutenin promoter (y-HGP). The length of the amplified y-HGP sequences ranged from 845 to 915 base pairs (bp) in the 25 species of Triticeae. Multiple sequence alignment showed conserved and variable parts in the y-HGP sequences. Higher sequence conservation was detected in the regulatory elements of y-HGP. An 85-bp deletion was found in eight species of Triticum, Aegilops, and Hordeum. Several species-specific indels were identified in the y-HGP from Psathyrostachys, Hordeum, and Pseudoroegneria. Maximum parsimony (MP) and Bayesian analyses defined an Aegilops/Triticum group consisting of closely related species. A close relationship between Pseudoroegneria and the clade of Australopyrum, Dasypyrum, and Agropyron was also strongly supported in the topologies of MP and Bayesian trees. As y-HGP has sufficient amounts of genetic variation and is a single-copy region in diploid Triticeae, it is useful in phylogenetic analyses of this group.

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Acknowledgments

The authors thank Prof. Bernard R. Baum and Dr. John Lu of Agriculture and Agri-Food Canada for critical review of the manuscript. This work was supported by the National High Technology Research and Development Program of China (863 program 2006AA10Z179 and 2006AA10Z1F8), the National Basic Research Program (973 Program 2009CB118300), and the FANEDD project (200357) from Ministry of Education, China.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Ya’an, 625014, Sichuan, China

    Qian-Tao Jiang, Yu-Ming Wei, Ji-Rui Wang, Ze-Hong Yan & You-Liang Zheng

  2. Lethbridge Research Centre, Agri and Agri-Food, Lethbridge, T1J 4B1, Canada

    Qian-Tao Jiang

  3. Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, Ya’an, 625014, Sichuan, China

    You-Liang Zheng

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  1. Qian-Tao Jiang
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  2. Yu-Ming Wei
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  3. Ji-Rui Wang
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  4. Ze-Hong Yan
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Correspondence to You-Liang Zheng.

Additional information

Q.-T. Jiang and Y.-M. Wei contributed equally to this paper.

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606_2009_263_MOESM1_ESM.tif

Multiple sequence alignment of y-HGP from 25 diploid species of Triticeae. The species-specific indels are indicated by boxes. a and b, c and d, and e and f represent unique indels in Psathyrostachys, Pseudoroegneria, and Hordeum (TIFF 301 kb)

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Jiang, QT., Wei, YM., Wang, JR. et al. Molecular diversity and phylogenetic analyses of y-type high-molecular-weight glutenin promoters from different genomes in Triticeae. Plant Syst Evol 285, 131–138 (2010). https://doi.org/10.1007/s00606-009-0263-8

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  • DOI: https://doi.org/10.1007/s00606-009-0263-8

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