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Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars

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Summary.

Porcine circoviruses (PCV) are present in pigs worldwide; they are grouped into two types: PCV1 comprising non-pathogenic viruses and PCV2 responsible for several clinical manifestations. Both types are frequently detected in domestic pigs, the prevalence and role of PCV in wild boars however, is not well studied. During the years 2002–2003 over 2000 organ samples of Hungarian wild boars were collected, grouped and samples from 307 different animals were tested by polymerase chain reaction for the presence of PCV. 35.5% of the wild boars were positive for one or both PCV types and PCV2 was detected in 20.5% of the animals. The PCV2 viruses were divided into 7 groups (WB-H1-7) based on sequencing data and genomes representing these groups were sequenced completely. The wild boar PCV2 groups were distributed evenly in the geographical region, regardless of the time and place of collection. The phylogenetic analysis of the PCV2 sequences of wild boar and domestic pig origin showed the possibility of an epidemiological link between wild boar and domestic pig infections. Interestingly, the complete nucleotide sequence of the viruses and the predicted amino acid sequence of the replication associated protein (ORF1) grouped the viruses similarly, whereas the capsid protein (ORF2) comparisons revealed different relations among the groups, suggesting the possibility of genomic recombination in PCV2.

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References

  • G Allan B Meehan D Todd S Kennedy F McNeilly J Ellis EG Clark J Harding E Espuna A Botner C Charreyre (1998) ArticleTitleNovel porcine circoviruses from pigs with wasting disease syndromes. Vet Rec 142 467–468 Occurrence Handle1:STN:280:DyaK1c3msFahuw%3D%3D Occurrence Handle9602519

    CAS  PubMed  Google Scholar 

  • AK Cheung (2004) ArticleTitlePalindrome regeneration by template strand-switching mechanism at the origin of replication of porcine circovirus via the rolling-circle melting-pot replication model. J Virol 78 9016–9029 Occurrence Handle1:CAS:528:DC%2BD2cXntVKiurg%3D Occurrence Handle15308698

    CAS  PubMed  Google Scholar 

  • EG Clark (1997) ArticleTitlePost-weaning multisystemic wasting syndrome. Proc Am Ass Swine Pract 10 499–501

    Google Scholar 

  • L Darwich J Segalés E Mateu (2004) ArticleTitlePathogenesis of postweaning multisystemic wasting syndrome caused by Porcine circovirus 2: an immune riddle. Arch Virol 149 857–874 Occurrence Handle10.1007/s00705-003-0280-9 Occurrence Handle1:CAS:528:DC%2BD2cXjt1Sgsbc%3D Occurrence Handle15098103

    Article  CAS  PubMed  Google Scholar 

  • Á Dán T Molnár I Biksi R Glávits M Shaheim B Harrach (2003) ArticleTitleCharacterisation of Hungarian porcine circovirus 2 genomes associated with PMWS and PDNS cases. Acta Vet Hung 51 551–562 Occurrence Handle14680067

    PubMed  Google Scholar 

  • C de Boisseson V Beven L Bigarre R Thiery N Rose E Eveno F Madec A Jestin (2004) ArticleTitleMolecular characterization of Porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs. J Gen Virol 85 293–304 Occurrence Handle10.1099/vir.0.19536-0 Occurrence Handle1:CAS:528:DC%2BD2cXitVGit70%3D Occurrence Handle14769887

    Article  CAS  PubMed  Google Scholar 

  • J Ellis L Hassard E Clark J Hardling G Allan P Wilson J Strokappe K Martin F McNeilly B Meehan D Todd D Haines (1998) ArticleTitleIsolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can Vet J 39 44–51 Occurrence Handle1:STN:280:DyaK1c7gsFWlsQ%3D%3D Occurrence Handle9442952

    CAS  PubMed  Google Scholar 

  • J Ellis M Spinato C Yong K West F McNeilly B Meehan S Kennedy E Clark S Krakowka G Allan (2003) ArticleTitlePorcine circovirus 2-associated disease in Eurasian wild boar. J Vet Diagn Invest 15 364–368 Occurrence Handle12918819

    PubMed  Google Scholar 

  • AL Hamel LL Lin C Sachvie E Grudeski GP Nayar (2000) ArticleTitlePCR detection and characterization of type-2 porcine circovirus. Can J Vet Res 64 44–52 Occurrence Handle1:CAS:528:DC%2BD3cXhtVWgs74%3D Occurrence Handle10680656

    CAS  PubMed  Google Scholar 

  • L Heath DP Martin L Warburton M Perrin W Horsfield C Kingsley EP Rybicki A Williamson (2004) ArticleTitleEvidence of unique genotypes of Beak and Feather disease virus in Southern Africa. J Virol 78 9277–9284 Occurrence Handle10.1128/JVI.78.17.9277-9284.2004 Occurrence Handle1:CAS:528:DC%2BD2cXntVKhs74%3D Occurrence Handle15308722

    Article  CAS  PubMed  Google Scholar 

  • I Kiss S Kecskeméti T Tuboly E Bajmócy J Tanyi (2000) ArticleTitleNew pig disease in Hungary: postweaning multisystemic wasting syndrome caused by circovirus. Acta Vet Hung 48 469–475 Occurrence Handle1:STN:280:DC%2BD3MzjsFCktg%3D%3D Occurrence Handle11402663

    CAS  PubMed  Google Scholar 

  • S Krakowka JA Ellis F McNeilly S Ringler DM Rings G Allan (2001) ArticleTitleActivation of the immune system is the pivotal event in the production of wasting disease in pigs infected with porcine circovirus-2 (PCV-2). Vet Pathol 38 31–42 Occurrence Handle10.1354/vp.38-1-31 Occurrence Handle1:STN:280:DC%2BD3M7nslCgsA%3D%3D Occurrence Handle11199162

    Article  CAS  PubMed  Google Scholar 

  • SC Kyriakis K Saoulidis S Lekkas ChC Miliotis PA Papoutsis S Kennedy (2002) ArticleTitleThe effects of immuno-modulation on the clinical and pathological expression of postweaning multisystemic wasting syndrome. J Comp Pathol 126 38–46 Occurrence Handle10.1053/jcpa.2001.0520 Occurrence Handle1:STN:280:DC%2BD387isVertA%3D%3D Occurrence Handle11814320

    Article  CAS  PubMed  Google Scholar 

  • R Larochelle M Antaya M Morin R Magar (1999) ArticleTitleTyping of porcine circovirus in clinical specimens by multiplex PCR. J Virol Methods 80 69–75 Occurrence Handle10.1016/S0166-0934(99)00032-4 Occurrence Handle1:CAS:528:DyaK1MXjsVSht7w%3D Occurrence Handle10403678

    Article  CAS  PubMed  Google Scholar 

  • R Magar P Muller R Larochelle (2000) ArticleTitleExperimental transmission of porcine circovirus type 2 (PCV2) in weaned pigs: a sequential study. J Comp Pathol 123 258–269 Occurrence Handle10.1053/jcpa.2000.0413 Occurrence Handle1:STN:280:DC%2BD3M%2Fps12huw%3D%3D Occurrence Handle11041995

    Article  CAS  PubMed  Google Scholar 

  • A Mankertz M Domingo JM Folch P LeCann A Jestin J Segalés B Chmielewicz J Plana-Duran D Soike (2000) ArticleTitleCharacterisation of PCV-2 isolates from Spain, Germany and France. Virus Res 66 65–77 Occurrence Handle10.1016/S0168-1702(99)00122-7 Occurrence Handle1:CAS:528:DC%2BD3cXosVGmsw%3D%3D Occurrence Handle10653918

    Article  CAS  PubMed  Google Scholar 

  • T Molnár R Glávits L Szeredi Á Dán (2002) ArticleTitleOccurrence of porcine dermatitis and nephropathy syndrome in Hungary. Acta Vet Hung 50 5–16 Occurrence Handle12061235

    PubMed  Google Scholar 

  • I Morozov T Sirinarumitr SD Sorden PG Halbur MK Morgan KJ Yoon PS Paul (1998) ArticleTitleDetection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J Clin Microbiol 36 2535–2541 Occurrence Handle1:CAS:528:DyaK1cXlvVehsb4%3D Occurrence Handle9705388

    CAS  PubMed  Google Scholar 

  • FD Niagro AN Forsthoefel RP Lawther L Kamalanathan BW Ritchie KS Latimer PD Lukert (1998) ArticleTitleBeak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. Arch Virol 143 1723–1744 Occurrence Handle10.1007/s007050050412 Occurrence Handle1:CAS:528:DyaK1cXms1Ggsrw%3D Occurrence Handle9787657

    Article  CAS  PubMed  Google Scholar 

  • B O’Connor H Gauvreau K West J Bogdan M Ayroud EG Clark C Konoby G Allan JA Ellis (2001) ArticleTitleMultiple porcine circovirus 2-associated abortions and reproductive failure in a multisite swine production unit. Can Vet J 42 551–553 Occurrence Handle1:STN:280:DC%2BD3MvhvVyjtg%3D%3D Occurrence Handle11467184

    CAS  PubMed  Google Scholar 

  • M Ouardani L Wilson R Jette C Montpetit SJ Dea (1999) ArticleTitleMultiplex PCR for detection and typing of porcine circoviruses. Clin Microbiol 37 3917–3924 Occurrence Handle1:CAS:528:DyaK1MXotV2nurs%3D

    CAS  Google Scholar 

  • C Rosell J Segalés J Plana-Duran M Balasch GM Rodriguez-Arrioja S Kennedy GM Allan F McNeilly KS Latimer M Domingo (1999) ArticleTitlePathological, immunohistochemical, and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. J Comp Pathol 120 59–78 Occurrence Handle10.1053/jcpa.1998.0258 Occurrence Handle1:STN:280:DyaK1M7pvVSruw%3D%3D Occurrence Handle10098016

    Article  CAS  PubMed  Google Scholar 

  • C Rosell J Segalés JA Ramos-Vara JM Folch GM Rodriguez-Arrioja CO Duran M Balasch J Plana-Duran M Domingo (2000) ArticleTitleIdentification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome. Vet Rec 146 40–43 Occurrence Handle1:STN:280:DC%2BD3c7kt1aktQ%3D%3D Occurrence Handle10678809

    CAS  PubMed  Google Scholar 

  • Sanchez R, Nauwynck H, Pensaert M (2001) Serological survey of porcine circovirus 2 antibodies in domestic and feral pig populations in Belgium. Proc. European Society for Veterinary Virology: ssDNA viruses of plants, birds, pigs and primates. St Malo, France

  • K Sato T Shibahara Y Ishikawa H Kondo M Kubo K Kadota (2000) ArticleTitleEvidence of porcine circovirus infection in pigs with wasting disease syndrome from 1985 to 1999 in Hokkaido, Japan. J Vet Med Sci 62 627–633 Occurrence Handle1:STN:280:DC%2BD3M%2FjtVSrsQ%3D%3D Occurrence Handle10907690

    CAS  PubMed  Google Scholar 

  • C Schulze J Segalés G Neumann A Hlinak M Calsamiglia M Domingo (2004) ArticleTitleIdentification of postweaning multisystemic wasting syndrome in European wild boar (Sus scrofa). Vet Rec 154 694–696 Occurrence Handle1:STN:280:DC%2BD2c3pvV2hsA%3D%3D Occurrence Handle15200077

    CAS  PubMed  Google Scholar 

  • Segalés J, Marco I, Calsamiglia M, Rodriguez-Arrioja GM, Lavin S, Plana-Duran J, Domingo M (2002) Evidence of porcine circovirus type 2 infection in wild boar (Sus scrofa) in Spain. Proc. 17th International Pig Veterinary Society Congress. Ames, USA

  • J Segalés M Domingo (2002) ArticleTitlePostweaning multisystemic wasting syndrome (PMWS) in pigs. Vet Quart 24 109–124

    Google Scholar 

  • GW Stevenson M Kiupel SK Mittal J Choi KS Latimer CL Kanitz (2001) ArticleTitleTissue distribution and genetic typing of porcine circoviruses in pigs with naturally occurring congenital tremors. J Vet Diagn Invest 13 57–62 Occurrence Handle1:STN:280:DC%2BD3M3osFOgug%3D%3D Occurrence Handle11243364

    CAS  PubMed  Google Scholar 

  • I Tischer R Rasch G Tochtermann (1974) ArticleTitleCharacterization of papovavirus- and picornavirus- like particles in permanent pig kidney cell lines. Zbl Bakteriol A 226 153–167 Occurrence Handle1:STN:280:CSuC2MbhtlU%3D

    CAS  Google Scholar 

  • I Tischer H Gelderblom W Vetterman MA Koch (1982) ArticleTitleA very small porcine virus with circular single-stranded DNA. Nature 295 64–66 Occurrence Handle10.1038/295064a0 Occurrence Handle1:CAS:528:DyaL38XhvFKhs7g%3D Occurrence Handle7057875

    Article  CAS  PubMed  Google Scholar 

  • I Tischer W Mields D Wolff M Vagt W Griem (1986) ArticleTitleStudies on epidemiology and pathogenicity of porcine circovirus. Arch Virol 91 271–276 Occurrence Handle10.1007/BF01314286 Occurrence Handle1:STN:280:BiiD2cfhsVw%3D Occurrence Handle3778212

    Article  CAS  PubMed  Google Scholar 

  • I Toplak J Grom P Hostnik D Barlic-Maganja (2004) ArticleTitlePhylogenetic analysis of type 2 porcine circoviruses identified in wild boar in Slovenia. Vet Rec 155 178–180 Occurrence Handle1:STN:280:DC%2BD2cvkvFGhsQ%3D%3D Occurrence Handle15357380

    CAS  PubMed  Google Scholar 

  • J Vicente J Segalés U Hofle M Balasch J Plana-Duran M Domingo C Gortazar (2004) ArticleTitleEpidemiological study on porcine circovirus type 2 (PCV2) infection in the European wild boar (Sus scrofa). Vet Res 35 243–253 Occurrence Handle10.1051/vetres:2004008 Occurrence Handle15099500

    Article  PubMed  Google Scholar 

  • E Wattrang F McNeilly GM Allan C Greko C Fossum P Wallgren (2002) ArticleTitleExudative epidermitis and porcine circovirus-2 infection in a Swedish SPF-herd. Vet Microbiol 86 281–293 Occurrence Handle10.1016/S0378-1135(02)00024-X Occurrence Handle11955778

    Article  PubMed  Google Scholar 

  • GJ Wellenberg N Stockhofe-Zurwieden MF De Jong WJA Boersma ARW Elbers (2004) ArticleTitleExcessive porcine circovirus type 2 antibody titres may trigger the development of porcine dermatitis and nephropathy syndrome: a case-control study. Vet Microbiol 99 203–214 Occurrence Handle10.1016/j.vetmic.2004.01.001 Occurrence Handle1:STN:280:DC%2BD2c7ntlGnsg%3D%3D Occurrence Handle15066723

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology and Infectious Diseases, Szent István University, Faculty of Veterinary Science, Budapest, Hungary

    A. Cságola & T. Tuboly

  2. Central Veterinary Institute, Institute of Debrecen, Debrecen, Hungary

    S. Kecskeméti, G. Kardos & I. Kiss

  3. Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary

    G. Kardos

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  1. A. Cságola
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  2. S. Kecskeméti
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  3. G. Kardos
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  4. I. Kiss
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  5. T. Tuboly
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Cságola, A., Kecskeméti, S., Kardos, G. et al. Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars. Arch Virol 151, 495–507 (2006). https://doi.org/10.1007/s00705-005-0639-1

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  • DOI: https://doi.org/10.1007/s00705-005-0639-1

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