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Genetic diversity of Brazilian strains of porcine circovirus type 2 (PCV-2) revealed by analysis of the cap gene (ORF-2)

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Summary

Porcine circovirus 2 (PCV-2) is associated with a broad range of syndromes. In this study, 19 of 870 samples from pigs from different Brazilian states were found to be positive for PCV-2 by polymerase chain reaction (PCR). A fragment of 700 nt of the cap gene (ORF-2) from the 19 PCV-2-positive samples were sequenced using three pairs of primers (Fa/Ra, Fb/Rb and Fc/Rc). Maximum parsimony genealogy with a heuristic algorithm using the 19 field strain studied here, 21 sequences from GenBank and PCV-1 as an out-group showed the existence of two major clusters (A and B) and the Brazilian strains segregating in both of them. PCV-2 was found in pigs with various clinical signs. No association between clusters of PCV-2 and different states or clinical signs were observed, demonstrating that the exact role of PCV-2 in porcine circovirus diseases (PCVD) in Brazil still needs to be clarified. These results contribute to the molecular characterization of PCV-2, which serve as a basis for the epimiology of PCV-2 infection.

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Authors and Affiliations

  1. Faculdade de Jaguariúna, Curso de Medicina Veterinária, Jaguariúna, São Paulo, Brazil

    A. M. Martins Gomes de Castro

  2. Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil

    A. Cortez, P. E. Brandão & L. J. Richtzenhain

  3. Escola de Veterinária, Universidade Federal de Minas Gerais, Minas Gerais, Brazil

    M. B. Heinemann

  4. Circovirus Research Group, São Paulo, Brazil

    A. M. Martins Gomes de Castro, P. E. Brandão & L. J. Richtzenhain

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  1. A. M. Martins Gomes de Castro
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  2. A. Cortez
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  3. M. B. Heinemann
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  4. P. E. Brandão
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  5. L. J. Richtzenhain
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Martins Gomes de Castro, A., Cortez, A., Heinemann, M. et al. Genetic diversity of Brazilian strains of porcine circovirus type 2 (PCV-2) revealed by analysis of the cap gene (ORF-2). Arch Virol 152, 1435–1445 (2007). https://doi.org/10.1007/s00705-007-0976-3

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