Abstract
Alveolar macrophages from PRRSV-infected and naïve pigs were placed into culture and infected with PRRSV laboratory strain SD-23983. Permissiveness increased with time in culture, and macrophages from infected pigs could be superinfected. Addition of actinomycin D, an inhibitor of mRNA synthesis, blocked infection. Interferon-γ reduced infection in cultures, while the addition of tumor necrosis factor-α or interleukin (IL)-10 did not affect permissiveness. IL-4 produced a marginal increase in the percentage of infected cells, but without a detectable increase in virus yield. These results suggest that the PRRSV-permissive population of cells in culture arises from a non-permissive precursor population and depends on new mRNA synthesis.
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Acknowledgments
The authors thank Paula Schneider and John Patton for collecting and processing PAMs, Megan Rolf for assistance with the cytokine assays, and Eric Nelson, South Dakota State University, Brookings, for generously providing the SDOW-17 antibody. This work was supported by National Science Foundation Grant No. SBE-0244984, the Kansas Agricultural Experiment Station #08-21-J, United States Department of Agriculture (USDA) Cooperative State Research, Education and Extension Service, grant number 2003-35204-13704 and the USDA National Research Initiative PRRS Integrated Program Project.
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Gaudreault, N., Rowland, R.R.R. & Wyatt, C.R. Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus. Arch Virol 154, 133–136 (2009). https://doi.org/10.1007/s00705-008-0271-y
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DOI: https://doi.org/10.1007/s00705-008-0271-y