Abstract
MicroRNAs are key players in the regulation of gene expression by posttranscriptional suppression. They are involved in physiological processes, and thus their deregulation may contribute to the development of diseases and progression of cancer. Virus-encoded microRNAs and microRNAs of host origin play an important role in controlling the virus life cycle and immunity. The aim of this study was to determine the effect of vaccinia virus (VACV) infection on the expression of host-encoded microRNAs. A marked general suppression of most microRNAs in the infected cells was observed within 24 hours after VACV infection of a number of cell types. We demonstrate that this suppression was associated with abrogation of expression of the Dicer1 enzyme, which is a key enzyme in the generation of microRNAs.
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Acknowledgment
This work was supported by the Israel Science Foundation (grant no. 1375/05) and the Israel Ministry for Science, Culture, and Sport (grant no. 3-4435). The sponsor had no role in the study.
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Supplementary material 1 (PPTX 55 kb)Supplemental Figure 1 Abrogation of Dicer1 in a variety of VACV-infected cells. HeLa, Vero and 293 cells were infected with VACV for 48 h (white bars) or mock-infected (black bars). Following infection, total RNA was extracted, and SYBR green RT-PCR was performed to measure transcription of Drosha (panel a) and Dicer1 (panel b) mRNAs. While Dicer1 expression was abolished in infected cells (panel b), there was no difference in Drosha expression in infected vs. uninfected cells (panel a). The average of the data from two experiments is shown
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Grinberg, M., Gilad, S., Meiri, E. et al. Vaccinia virus infection suppresses the cell microRNA machinery. Arch Virol 157, 1719–1727 (2012). https://doi.org/10.1007/s00705-012-1366-z
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DOI: https://doi.org/10.1007/s00705-012-1366-z