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Detection of Norwalk-like virus genes in the caecum contents of pigs

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Summary

Norwalk-like virus genes were detected by RT-PCR in the caecum contents of pigs. Positive PCR products were produced from four out of 1 117 samples by nested PCR using human SRSV primers. Nucleotide sequences between 4 561 and 4 852 numbered according to the Norwalk virus genomic RNA in the RNA polymerase region were determined. Between the Norwalk virus sequence and the sequences detected in pigs, there was 58.2% to 59.9% sequence homology. The swine sequences were located on genogroup II of human SRSVs, but formed a subgroup in the phylogenetic tree of caliciviruses.

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Authors and Affiliations

  1. Shizuoka Prefectural Institute of Public Health and Environmental Science, Shizuoka-shi, Shizuoka, Japan, Japan

    M. Sugieda, H. Nagaoka, Y. Kakishima, T. Ohshita & S. Nakamura

  2. Department of Microbiology, National Institute of Public Health, Minato-ku, Tokyo, Japan, Japan

    S. Nakajima

Authors
  1. M. Sugieda
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  2. H. Nagaoka
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  3. Y. Kakishima
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  4. T. Ohshita
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  5. S. Nakamura
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  6. S. Nakajima
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Received December 15, 1997 Accepted January 27, 1998

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Sugieda, M., Nagaoka, H., Kakishima, Y. et al. Detection of Norwalk-like virus genes in the caecum contents of pigs. Arch. Virol. 143, 1215–1221 (1998). https://doi.org/10.1007/s007050050369

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  • DOI: https://doi.org/10.1007/s007050050369

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