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Arabidopsis thaliana Somatic Embryogenesis Receptor Kinase 1 protein is present in sporophytic and gametophytic cells and undergoes endocytosis

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Summary.

Arabidopsis thaliana plants expressing AtSERK1 fused to yellow-fluorescent protein were generated. Fluorescence was detected predominantly at the cell periphery, most likely the plasma membrane, of cells in ovules, embryo sacs, anthers, and embryos and in seedlings. The AtSERK1 protein was detected in diverse cell types including the epidermis and the vascular bundles. In some cells, fluorescent receptors were seen in small vesicle-like compartments. After application of the fungal toxin Brefeldin A, the fluorescent receptors were rapidly internalized in the root meristem and root vascular tissue. We conclude that the AtSERK1 receptor functions in a common signalling pathway employed in both sporophytic and gametophytic cells.

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Abbreviations

BFA:

Brefeldin A

CHX:

cycloheximide

CLSM:

confocal laser scanning microscopy

LRR:

leucine-rich repeat

MMC:

megaspore mother cell

RLK:

receptor-like kinase

YFP:

yellow-fluorescent protein

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Authors and Affiliations

  1. Laboratory of Biochemistry, Wageningen University, Wageningen

    M. A. C. J. Kwaaitaal, S. C. de Vries & E. Russinova

Authors
  1. M. A. C. J. Kwaaitaal
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  2. S. C. de Vries
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  3. E. Russinova
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Corresponding author

Correspondence to E. Russinova.

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Correspondence and reprints: Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands.

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Kwaaitaal, M., de Vries, S. & Russinova, E. Arabidopsis thaliana Somatic Embryogenesis Receptor Kinase 1 protein is present in sporophytic and gametophytic cells and undergoes endocytosis. Protoplasma 226, 55–65 (2005). https://doi.org/10.1007/s00709-005-0111-9

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  • DOI: https://doi.org/10.1007/s00709-005-0111-9

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