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Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength

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Summary.

This study examined 10 wks of resistance training and the ingestion of supplemental protein and amino acids on muscle performance and markers of muscle anabolism. Nineteen untrained males were randomly assigned to supplement groups containing either 20 g protein (14 g whey and casein protein, 6 g free amino acids) or 20 g dextrose placebo ingested 1 h before and after exercise for a total of 40 g/d. Participants exercised 4 times/wk using 3 sets of 6–8 repetitions at 85–90% of the one repetition maximum. Data were analyzed with two-way ANOVA (p < 0.05). The protein supplement resulted in greater increases in total body mass, fat-free mass, thigh mass, muscle strength, serum IGF-1, IGF-1 mRNA, MHC I and IIa expression, and myofibrillar protein. Ten-wks of resistance training with 20 g protein and amino acids ingested 1 h before and after exercise is more effective than carbohydrate placebo in up-regulating markers of muscle protein synthesis and anabolism along with subsequent improvements in muscle performance.

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References

  • G Adams (1998) ArticleTitleRole of insulin-like growth factor-I in the regulation of skeletal muscle adaptation to increased loading Exerc Sport Sci Rev 26 31–60 Occurrence Handle9696984 Occurrence Handle10.1249/00003677-199800260-00004 Occurrence Handle1:STN:280:DyaK1czmt1eisg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • InstitutionalAuthorNameAmerican College of Sports Medicine (2000) Guidelines for exercise testing and prescription EditionNumber6 Lippincott Williams and Wilkins New York 24–26

    Google Scholar 

  • P Andersen B Saltin (1985) ArticleTitleMaximal perfusion of skeletal muscle in man J Physiol 366 233–249 Occurrence Handle4057091 Occurrence Handle1:STN:280:BimD3snos1Q%3D

    PubMed  CAS  Google Scholar 

  • L Andersen G Tufekovic M Zebis R Crameri G Verlaan M Khaer C Suetta P Magnusson P Aagaard (2005) ArticleTitleThe effect of resistance training combined with timed ingestion of protein on muscle fiber size and muscle strength Metabolism 54 151–156 Occurrence Handle15690307 Occurrence Handle10.1016/j.metabol.2004.07.012 Occurrence Handle1:CAS:528:DC%2BD2MXos1OrtA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • T Baechle R Earle D Wathen (2000) Resistance training T Baechle R Earle (Eds) Essentials of strength training and conditioning EditionNumber2 Human Kinetic Champaign, IL 420–421

    Google Scholar 

  • T Ballard J Clapper B Specker T Binkley M Vukovich (2005) ArticleTitleEffect of protein supplementation during a 6-mo strength and conditioning program on insulin-like growth factor I and markers of bone turnover in young adults Am J Clin Nutr 81 1442–1448 Occurrence Handle15941900 Occurrence Handle1:CAS:528:DC%2BD2MXlsVWiu7w%3D

    PubMed  CAS  Google Scholar 

  • M Bamman J Shipp J Jiang B Gower G Hunter A Goodman C McLafferty R Urban (2001) ArticleTitleMechanical load increase muscle IGF-1 and androgen receptor mRNA concentrations in humans Am J Physiol Endocrinol Metab 280 E383–E390 Occurrence Handle11171591 Occurrence Handle1:CAS:528:DC%2BD3MXjtVWru7k%3D

    PubMed  CAS  Google Scholar 

  • G Biolo K Tipton S Klein R Wolfe (1997) ArticleTitleAn abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein Am J Physiol 273 E122–E129 Occurrence Handle9252488 Occurrence Handle1:CAS:528:DyaK2sXlt1Srsb4%3D

    PubMed  CAS  Google Scholar 

  • S Bird K Tarpenning K Marino (2006) ArticleTitleIndependent and combined effects of liquid carbohydrate/essential amino acid ingestion on hormonal and muscular adaptations following resistance training in untrained men Eur J Appl Physiol 3 1–14

    Google Scholar 

  • D Bolster L Jefferson S Kimball (2004) ArticleTitleRegulation of protein synthesis associated with skeletal muscle hypertrophy by insulin-, amino acid-, and exercise-induced signaling Proc Nutr Soc 63 351–356 Occurrence Handle15294054 Occurrence Handle10.1079/PNS2004355 Occurrence Handle1:CAS:528:DC%2BD2cXms12msbY%3D

    Article  PubMed  CAS  Google Scholar 

  • E Borsheim A Aarsland R Wolfe (2004a) ArticleTitleEffect of an amino acid, protein, and carbohydrate mixture on net muscle protein balance after resistance exercise Int J Sport Nutr Exerc Metab 14 255–271 Occurrence Handle1:CAS:528:DC%2BD2cXnvVGntr4%3D

    CAS  Google Scholar 

  • E Borsheim M Cree K Tipton T Elliott A Aarsland R Wolfe (2004b) ArticleTitleEffect of carbohydrate intake of net protein synthesis during recovery from resistance exercise J Appl Physiol 96 674–678 Occurrence Handle10.1152/japplphysiol.00333.2003 Occurrence Handle1:CAS:528:DC%2BD2cXhsVyjsLk%3D

    Article  CAS  Google Scholar 

  • J Bowtell G Leese K Smith P Watt A Nevill O Rooyackers A Wagenmakers M Rennie (2000) ArticleTitleEffect of oral glucose on leucine turnover in human subjects at rest and during exercise at two levels of dietary protein J Physiol 525 271–281 Occurrence Handle10811743 Occurrence Handle10.1111/j.1469-7793.2000.00271.x Occurrence Handle1:CAS:528:DC%2BD3cXjvFGisrw%3D

    Article  PubMed  CAS  Google Scholar 

  • M Bradford (1976) ArticleTitleA rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein dye binding Anal Biochem 72 248–254 Occurrence Handle942051 Occurrence Handle10.1016/0003-2697(76)90527-3 Occurrence Handle1:CAS:528:DyaE28XksVehtrY%3D

    Article  PubMed  CAS  Google Scholar 

  • C Carroll J Fluckey R Williams D Sullivan T Trappe (2005) ArticleTitleHuman solues and vastus lateralis muscle protein metabolism with an amino acid infusion Am J Physiol Endocrinol Metab 288 E479–E485 Occurrence Handle15507532 Occurrence Handle10.1152/ajpendo.00393.2004 Occurrence Handle1:CAS:528:DC%2BD2MXisVKqsrs%3D

    Article  PubMed  CAS  Google Scholar 

  • P Chomczynski N Sacchi (1987) ArticleTitleSingle-step method of RNA isolation by acid guanidium thiocynate-phenol-chloroform extraction Anal Biochem 162 156–159 Occurrence Handle2440339 Occurrence Handle10.1016/0003-2697(87)90021-2 Occurrence Handle1:CAS:528:DyaL2sXitFSns7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • Current Protocols in Molecular Biology (1999) Short protocols in molecular biology. Wiley, New York, pp A3: 10–11

  • M Dangin Y Boirie C Garcia-Rodenas P Gachon J Fauquant P Gallier O Ballevre B Beaufrere (2001) ArticleTitleThe digestion rate of protein is an independent regulating factor of postprandial protein retention Am J Physiol Endocrinol Metab 280 E340–E348 Occurrence Handle11158939 Occurrence Handle1:CAS:528:DC%2BD3MXjtVajs70%3D

    PubMed  CAS  Google Scholar 

  • M Hameed K Lange J Andersen P Schjerling M Kjaer D Harridge G Goldspink (2003) ArticleTitleThe effect of recombinant growth hormone and resistance training on IGF-1 mRNA expression in the muscles of elderly men J Physiol 555 231–240 Occurrence Handle14565994 Occurrence Handle10.1113/jphysiol.2003.051722

    Article  PubMed  Google Scholar 

  • H Karlsson P Nilsson J Nilsson A Chibalin J Zierath E Blomstrand (2004) ArticleTitleBranched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise Am J Physiol Endocrinol Metab 287 E1–E7 Occurrence Handle14998784 Occurrence Handle10.1152/ajpendo.00430.2003 Occurrence Handle1:CAS:528:DC%2BD2cXlvFOhtLc%3D

    Article  PubMed  CAS  Google Scholar 

  • R Koopman A Wagenmakers R Manders A Zorenc J Senden M Gorselink H Keizer L van Loon (2005) ArticleTitleCombined ingestion of protein and free leucine with carbohydrate increases postexercise muscle protein synthesis in vivo in male subjects Am J Physiol Endocrinol Metab 288 E645–E653 Occurrence Handle15562251 Occurrence Handle10.1152/ajpendo.00413.2004 Occurrence Handle1:CAS:528:DC%2BD2MXjtlOqu7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • D Mahoney K Carey M Fu D Cameron-Smith G Parise M Tarnopolsky (2004) ArticleTitleReal-time RT-PCR analysis of housekeeping genes in human skeletal muscle following acute exercise Physiol Genomics 8 226–231

    Google Scholar 

  • S Phillips K Tipton A Aarsland S Wolf R Wolfe (1997) ArticleTitleMixed muscle protein synthesis and breakdown after resistance exercise in humans Am J Physiol 273 E99–E107 Occurrence Handle9252485 Occurrence Handle1:CAS:528:DyaK2sXlt1SrsLk%3D

    PubMed  CAS  Google Scholar 

  • N Psilander R Damsgaard H Pilegaard (2003) ArticleTitleResistance exercise alters MRG and IFG-1 mRNA content in human skeletal muscle J Appl Physiol 95 1038–1044 Occurrence Handle12716875 Occurrence Handle1:CAS:528:DC%2BD3sXnsFCjsbk%3D

    PubMed  CAS  Google Scholar 

  • B Rasmussen K Tipton S Miller S Wolf R Wolfe (2000) ArticleTitleAn oral essential amino acid-carbohydrate supplement enhances muscle protein anabolism after resistance exercise J Appl Physiol 88 386–392 Occurrence Handle10658002 Occurrence Handle1:CAS:528:DC%2BD3cXht12ju7c%3D

    PubMed  CAS  Google Scholar 

  • D Roy M Tarnopolsky J MacDougall J Fowles K Yarasheski (1997) ArticleTitleEffect of glucose supplement timing on protein metabolism after resistance training J Appl Physiol 82 1882–1888 Occurrence Handle9173954 Occurrence Handle10.1063/1.365993 Occurrence Handle1:CAS:528:DyaK2sXkt1Oqt74%3D

    Article  PubMed  CAS  Google Scholar 

  • D Roy J Fowles R Hill M Tarnopolsky (2000) ArticleTitleMacronutrient intake and whole body protein metabolism following resistance exercise Med Sci Sports Exerc 32 1412–1418 Occurrence Handle10949007 Occurrence Handle10.1097/00005768-200008000-00009 Occurrence Handle1:CAS:528:DC%2BD3cXmtFKisr8%3D

    Article  PubMed  CAS  Google Scholar 

  • V Sartorelli M Fulco (2004) ArticleTitleMolecular and cellular determinants of skeletal muscle atrophy and hypertrophy Sci STKE 244 re11 Occurrence Handle10.1126/stke.2442004re11

    Article  Google Scholar 

  • K Tipton T Elliott M Cree S Wolf A Sanford R Wolfe (2004) ArticleTitleIngestion of casein and whey proteins result in muscle anabolism after resistance exercise Med Sci Sports Exerc 36 2073–2081 Occurrence Handle15570142 Occurrence Handle10.1249/01.MSS.0000147582.99810.C5 Occurrence Handle1:CAS:528:DC%2BD2cXhtVals73F

    Article  PubMed  CAS  Google Scholar 

  • J Walberg-Rankin L Goldman M Puglisi S Nickols-Richardson C Earthman F Gwazdauskas (2004) ArticleTitleEffect of post-exercise supplement consumption on adaptations to resistance training J Am Coll Nutr 23 322–330

    Google Scholar 

  • S Welle K Bhatt C Thornton (1999) ArticleTitleStimulation of myofibrillar synthesis by exercise is mediated by more efficient translation of mRNA J Appl Physiol 86 1220–1225 Occurrence Handle10194206 Occurrence Handle1:CAS:528:DyaK1MXivVGlsbs%3D

    PubMed  CAS  Google Scholar 

  • D Willoughby (2004a) ArticleTitleEffects of an alleged myostatin-binding supplement and heavy resistance training on serum myostatin muscle strength and mass, and body composition Int J Sport Nutr Exerc Metab 14 461–472 Occurrence Handle1:CAS:528:DC%2BD2cXnvFajs7s%3D

    CAS  Google Scholar 

  • D Willoughby (2004b) ArticleTitleEffects of heavy resistance training on myostatin mRNA and protein expression Med Sci Sports Exerc 36 574–582 Occurrence Handle10.1249/01.MSS.0000121952.71533.EA

    Article  Google Scholar 

  • D Willoughby M Nelson (2002) ArticleTitleMyosin heavy-chain mRNA expression after a single session of heavy-resistance exercise Med Sci Sports Exerc 34 1262–1269 Occurrence Handle12165680 Occurrence Handle10.1097/00005768-200205001-01040 Occurrence Handle1:CAS:528:DC%2BD38Xms1SnurY%3D

    Article  PubMed  CAS  Google Scholar 

  • D Willoughby J Rosene (2001) ArticleTitleEffects of oral creatine and resistance training on myosin heavy chain expression Med Sci Sports Exerc 33 1674–1681 Occurrence Handle11581551 Occurrence Handle10.1097/00005768-200105001-00170 Occurrence Handle1:CAS:528:DC%2BD3MXnvVCksLw%3D

    Article  PubMed  CAS  Google Scholar 

  • S Yang M Alnaqeeb H Simpson J Goldspink (1996) ArticleTitleCloning and characterization of an IGF-1 isoform expressed in skeletal muscle subjected to stretch J Muscle Res Cell Motil 17 487–495 Occurrence Handle8884603 Occurrence Handle10.1007/BF00123364 Occurrence Handle1:CAS:528:DyaK28XmtFChsbg%3D

    Article  PubMed  CAS  Google Scholar 

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Willoughby, D., Stout, J. & Wilborn, C. Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength. Amino Acids 32, 467–477 (2007). https://doi.org/10.1007/s00726-006-0398-7

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  • DOI: https://doi.org/10.1007/s00726-006-0398-7

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