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Rapid detection of methicillin-resistant Staphylococcus aureus from screening enrichment broths by real-time PCR

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Abstract

The aim of this study was to evaluate real-time PCR (LightCycler) kits for the detection of methicillin-resistant Staphylococcus aureus in enrichment broth using 200 sets of patient screening swab samples. One hundred sets were processed using a lysostaphin/lysozyme extraction method and a further 100 were tested using a column extraction kit. The PCR kits with lysostaphin/lysozyme lysis showed 95.7% sensitivity, 90.8% specificity, and negative and positive predictive values of 98.6% and 75.9%, respectively, compared with 88%, 95.9%, 84.6% and 95.9%, respectively, with the column extraction kit.

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Acknowledgements

We are indebted to the staff at the Department of Microbiology at University Hospital, Nottingham, UK, for their collaboration in providing screening swabs and to Roche Diagnostics for providing the LightCycler kits

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Authors and Affiliations

  1. Department of Microbiology, Molecular Diagnostics and Typing Unit, University Hospital, Queen’s Medical Centre, Nottingham, NG7 2UH, UK

    K. Levi & K. J. Towner

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  1. K. Levi
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  2. K. J. Towner
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Correspondence to K. Levi.

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Levi, K., Towner, K.J. Rapid detection of methicillin-resistant Staphylococcus aureus from screening enrichment broths by real-time PCR. Eur J Clin Microbiol Infect Dis 24, 423–427 (2005). https://doi.org/10.1007/s10096-005-1336-4

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  • DOI: https://doi.org/10.1007/s10096-005-1336-4

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