Abstract
Four strains of marine stramenopilan protists, the thraustochytrids, were studied for their ability to produce extracellular polysaccharides (EPSs). Observations by light and scanning electron microscopy revealed the production of a matrix of EPS around groups of cells in stationary cultures. EPS in shake culture filtrates ranged from 0.3 to 1.1 g/L. EPS production, which was studied in greater detail in 2 isolates, SC-1 and CW1, increased with age of cultures, reaching a peak in the stationary phase. Anion exchange chromatography yielded a single fraction of the EPS of both species. The EPS contained 39% to 53% sugars, besides proteins, lipids, uronic acids, and sulfates. Molecular weight of the EPS produced by SC-1 was approximately 94 kDa, and that of CW1, 320 kDa. Glucose formed the major component in the EPS of both isolates—galactose, mannose, and arabinose being the other components. Cultures of both isolates survived air-drying up to a period of 96 hours, suggesting a role for EPS in preventing desiccation of cells.
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References
Bahnweg, G., Jäckle, I. (1986) “A new approach to taxonomy of the Thraustochytriales and Labyrinthulales” In: Moss, S.T. (ed.), The Biology of Marine Fungi, Cambridge University, Cambridge, U.K., pp 131-140
R.U. Bhat R.N. Tharanathan (1986) ArticleTitleFractionation of okra mucilage and structural investigation of an acidic polysaccharide Carbohyd Res 148 143–147 Occurrence Handle10.1016/0008-6215(86)80047-7
N.B. Bhosle A. Garg. S.S. Sawant A. Wagh (1995) ArticleTitleIsolation and partial chemical analysis of exopolysaccharides from the marine fouling diatoms Navicula subinflata Bot Mar 38 103–110
Bremer, G.B. (1976) “The ecology of marine lower fungi” In: Jones, E.B.G. (ed.), Recent Advances in Aquatic Mycology, Elek Science, London, U.K., pp 313-333
A.H.L. Chamberlain S.T. Moss (1986) ArticleTitleThe thraustochytrids: a protist group with mixed affinities Biosystems 21 341–349 Occurrence Handle10.1016/0303-2647(88)90031-7
B.E Christensen J. Kjosbakken O. Simdsrod (1985) ArticleTitlePartial chemical and physical characterization of two extracellular polysaccharides produced by marine, periphytic Pseudomonas sp strain NCMB 2021 Appl Environ Mirobiol 50 837–845
Colwell, R.R., Pariser, E.R., Sindkey, A.J. (eds.) (1986) Biotechnology of Marine Polysaccharides, Hemisphere Publishing Corporation, Washington
A.W. Decho (1990) ArticleTitleMicrobial exopolymer secretions in ocean environment: their role(s) in food webs and marine processes Oceanogr Mar Biol Annu Rev 28 73–153
Decho, A.W. (1993) “Methods for the observation and use in feeding experiments of microbial exopolymer” In: Kemp, P.F., Sherr, B.F., Sherr, E.B., Cole, J.J. (eds.), Handbook of Methods in Aquatic Microbial Ecology, Lewis Publishers, Chelsea, Mich., pp 685-694
Deming, J.M. (1993) “14C tracer method for measuring microbial activity in deep-sea sediments” In: Kemp, P.F., Sherr, B.F., Sherr, E.B., Cole, J.J. (eds.), Handbook of Methods in Aquatic Microbial Ecology, Lewis Publishers, Chelsea, Mich., pp 405-414
K.S. Dodgson (1961) ArticleTitleDetermination of inorganic sulphate in studies on the enzymatic and non-enzymatic hydrolysis of carbohydrate Exp Mar Biol Ecol 9 137–144
M. Dubois K.A. Gilles J.K. Hamilton P.A. Rebers F. Smith (1956) ArticleTitleColorimetric method for the determination of sugars and related substances Anal Chem 28 350–356 Occurrence Handle10.1021/ac60111a017
M.C.C. Filisetti-Cozzi N.C. Carpita (1991) ArticleTitleMeasurement of uronic acids without interference from neutral sugars Anal Biochem 107 157–162 Occurrence Handle10.1016/0003-2697(91)90372-Z
S. Geresh S. Arad (1991) ArticleTitleThe extracellular polysaccharides of the red microalgae: chemistry and rheology Bioresource Tecchnol 38 195–201 Occurrence Handle10.1016/0960-8524(91)90154-C
A. Gutierrez AT. Martinez A. Prieto (1996) ArticleTitleStructural characterization of extracellular polysaccharides produced by fungi from the genus Pleurotus Carbohydr Res 281 143–154 Occurrence Handle10.1016/0008-6215(95)00342-8 Occurrence Handle8839181
J. Jayaraman (2001) Laboratory Manual in Biochemistry New Age International (P) Limited, Publishers New Delhi, India 87
Khandeparkar, R.D.S., Bhosle, N.B. (2001) “Extracellular polymeric substances of the marine fouling diatom” Amphora rostrata Wm. Sm. Biofouling 17: 103-115
H. Kimura Y. Fukura T. Naganuma (1999) ArticleTitleBiomass of Thraustochytrid protoctists in coastal waters Mar Ecol Prog Ser 189 27–33
T.E. Lewis T.A. McMeekin P.D. Nicholas (1999) ArticleTitleThe biotechnological potential of thraustochytrids Mar Biotechnol 1 580–589 Occurrence Handle10612683
I. Majumdar N.B. Bhosle F. D’Souza (1999) ArticleTitleMicrobial exopolysaccharides: effect on corrosion and partial chemical characterization J Indian Inst Sci 79 539–550
T. Matsunaga H. Sudo H. Takemasa Y. Wachi N. Nakamura (1996) ArticleTitleSulfated extracellular polysaccharide production by the halophiliccyanobacterium Aphanocapsa halophytia immobilized on light-diffusers Appl Microbiol Biotechnol 45 24–27 Occurrence Handle10.1007/s002530050643
T. Naganuma H. Kimura H. Takasugi (1998) ArticleTitleAbundance of thraustochytrids in coastal plankton Mar Ecol Prog Ser 162 105–110
T.R. Parsons Y. Maita C.M. Lalli (1984) Manual of chemical and biological methods of seawater analysis Pergamon Press London, U.K.
G.L. Peterson (1977) ArticleTitleA simplification of the protein assay method of Lowry et al, which is more generally applicable Anal Biochem 83 346–356 Occurrence Handle10.1016/0003-2697(77)90043-4 Occurrence Handle603028
R.D. Philippis M. Vincenzini (1998) ArticleTitleExocellular polysaccharide from cyanobacteria and their possible applications FEMS Microbiol Rev 22 151–175 Occurrence Handle10.1016/S0168-6445(98)00012-6
R.D. Philippis M.C. Margheri R. Materassi M. Vincenzini (1998) ArticleTitlePotential of unicellular cyanobacteria from the saline environment as polysaccharide producers Appl Environ Microbiol 64 1130–1132
Porter, D. (1990) “Phylum Labyrinthulomycota” In: Margulis, L., Corliss, J.O., Melkonian, M., Chapman, D.J. (eds.), Handbook of Protoctista, Jones and Barlett, Boston, Mass., pp 388-398
S. Raghukumar (2002) ArticleTitleEcology of the marine protist, the Labyrinthulomycetes (Thraustochytrids and Labyrinthulids) Euro J Protistol 38 .
S. Raghukumar A.C. Anil L. Khandeparkar J.S. Patil (2000) ArticleTitleThraustochytrid protists as a component of marine microbial films MarBiol 136 603–609
S. Raghukumar C. Raghukumar N. Ramaiah (2001) ArticleTitleDynamics of thraustochytrid protists in the water column of the Arabian Sea Aquat Microb Ecol 24 175–186
K. Sogawa E. Kodama M. Matsuda K. Okutani S. Shigeta (1998) ArticleTitleMarine microalgal polysaccharides induces apoptosis in the human lymphoid cells J Mar Biotechnol 6 35–38
I.W. Sutherland (1985) ArticleTitleBiosynthesis and composition of gram negative bacterial extracellular and wall polysaccharides Annu Rev Microbiol 39 243–270 Occurrence Handle10.1146/annurev.mi.39.100185.001331 Occurrence Handle3904602
I.W. Sutherland (1998) ArticleTitleNovel and established applications of microbial polysaccharides Trends Biotechnol 16 41–46 Occurrence Handle10.1016/S0167-7799(97)01139-6 Occurrence Handle9470230
Tosteson, T.R. (1986) “The regulation and specificity of marine microbial surface interactions” In: Colwell, R.R. (ed.), Biotechnology of marine polysaccharides, Hemisphere Publishing Corp., Washington D.C., pp 115-130
A.J. Ulken G. Bahnweg (1985) ArticleTitleMorphology, nutrition and taxonomy of an Aplanochytrium sp. from the Sargasso Sea Mar Biol 85 89–95 Occurrence Handle10.1007/BF00396419
C. Whitfield (1988) ArticleTitleBacterial extracellular polysaccharides Can J Microbiol 34 415–420 Occurrence Handle3052752
Acknowledgments
One of us (R.J.) is grateful to the director, National Institute of Oceanography, support and encouragement. Laboratory facilities extended by the director, Central Food Technological Research Institute, are thankfully acknowledged. The cultures Mar3 and SR were kindly provided by Dr Lucia Bongiorni. This is NIO’s contribution 3888.
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Jain, R., Raghukumar, S., Tharanathan, R. et al. Extracellular Polysaccharide Production by Thraustochytrid Protists. Mar Biotechnol 7, 184–192 (2005). https://doi.org/10.1007/s10126-004-4025-x
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DOI: https://doi.org/10.1007/s10126-004-4025-x