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Phylogenetic Diversity and Distribution of Dissimilatory Sulfite Reductase Genes from Deep-Sea Sediment Cores

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Abstract

The diversity and distribution of sulfate-reducing prokaryotes (SRP) was investigated in the Nankai Trough sediments of off-central Japan by exploring the diversity of a functional gene, dissimilatory sulfite reductase (dsrAB). Bulk DNAs were extracted from five piston-cored samples (up to 4.5 m long) with 41 vertical sections, and full-length dsrABgene sequences (ca. 1.9 kb) were PCR amplified and cloned. A total of 382 dsrAB clones yielded eight phylogenetic groups with an indigenous group forming a unique dsrAB lineage. The deltaproteobacterial dsrAB genes were found in almost all sediment samples, especially in the surface layer. One unique dsrAB clone group was also widespread in the dsrAB profiles of the studied sediments, and the percentage of its clones was generally shown gradual increase with sediment depth.

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Acknowledgments

The authors thank the crew of RTV Bosei Maru, Tokai University, for sample collection. This work was supported by the Research Consortium for Methane Hydrate Resources (MH21) in Japan.

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Authors and Affiliations

  1. Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-hiroshima, 739-8528, Japan

    Ryo Kaneko, Toru Hayashi & Takeshi Naganuma

  2. Institute for Geo-Resources and Environment, National Institute of Advanced Industrial Science and Technology (AIST), AIST C-7, Tsukuba, 305-8567, Japan

    Manabu Tanahashi

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  1. Ryo Kaneko
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  2. Toru Hayashi
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  3. Manabu Tanahashi
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  4. Takeshi Naganuma
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Correspondence to Takeshi Naganuma.

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Kaneko, R., Hayashi, T., Tanahashi, M. et al. Phylogenetic Diversity and Distribution of Dissimilatory Sulfite Reductase Genes from Deep-Sea Sediment Cores. Mar Biotechnol 9, 429–436 (2007). https://doi.org/10.1007/s10126-007-9003-7

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