Abstract
The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.
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Acknowledgments
The authors thank Dr. Fran van Dolah (NOAA) and Kristy B. Lidie for donation of the K. brevis chloroplast clones, Mara Lennard for the donation of the catfish Bob1 clone, and Dr. Javier Robalino for the donation of the white spot syndrome virus clones. This paper is Marine Genome Project Contribution no. 30 and no. 618 of the South Carolina Department of Natural Resources. Part of the research (graduate research support for M.J. Jenny) was conducted under an award from the Estuarine Reserves Division, Office of Ocean and Coastal Resource Management, National Ocean Service, National Oceanic and Atmospheric Administration and the Science to Achieve Results Graduate Fellowship, U.S. Environmental Protection Agency. Construction and characterization of the Crassostrea cDNA microarray was also supported by National Oceanic and Atmospheric Administration Center of Excellence for Oceans and Human Health at the Hollings Marine Laboratory (National Ocean Service, National Centers for Coastal Ocean Science), as well as the Ernest F. Hollings Visiting Scholar Program (C. Cunningham). Additional funding came from the Oyster Disease Research Program, NOAA agreement no. NA16RG1039 and Sea GrantProject R/SAQ-08-NSI. The authors would also like to thank Drs. A.F. Holland and P.A. Sandifer for their encouragement and enthusiasm throughout all phases of the work. We thank Drs. Christopher Bayne, Philippe Roch, and Andrew Mount for careful reading of the manuscript and many helpful suggestions. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis (contact Paul Gross; grossp@musc.edu).
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Jenny, M.J., Chapman, R.W., Mancia, A. et al. A cDNA Microarray for Crassostrea virginica and C. gigas . Mar Biotechnol 9, 577–591 (2007). https://doi.org/10.1007/s10126-007-9041-1
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DOI: https://doi.org/10.1007/s10126-007-9041-1