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Suggestive Association of Major Histocompatibility IB Genetic Markers with Resistance to Bacterial Cold Water Disease in Rainbow Trout (Oncorhynchus mykiss)

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Abstract

Genes within the major histocompatibility complex (MHC) are important for both innate and adaptive immune responses in mammals; however, much less is known regarding their contribution in teleost fishes. We examined the involvement of four major histocompatibility (MH) genomic regions in rainbow trout in resistance to the causative agent of bacterial coldwater disease (BCWD), Flavobacterium psychrophilum. Fish from the 2005 NCCCWA brood-year (71 full-sib families) were challenged with F. psychrophilum strain CSF 259–93. The overall mortality rate was 70%, with large variation in mortality between families. Disease resistance was quantified as post-challenge days to death. Phenotypic variation and additive genetic variation were estimated using mixed models of survival analysis. To examine association, eight microsatellite markers were isolated from MH gene-containing BAC clones and mapped onto the rainbow trout genetic linkage map. The parents and grandparents of the 2005 brood-year families were genotyped with these eight markers and another two markers tightly linked to the MH-IB region to assess the extent of linkage disequilibrium (LD) of MH genomic regions MH-IA, MH-IB, TAP1, and MH-II with survival post-challenge. MH-IB and MH-II markers were linked to BCWD survivability when data were analyzed by family. Tests for disease association at the population level substantiated the involvement of MH-IB, but not MH-II, with disease resistance. The impact of selective breeding for disease resistance on MH sequence variation is discussed in the context of aquaculture production.

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Acknowledgments

The authors thank Dr. Caird Rexroad III, M. Renee Fincham, Melissa Johnson, Kristy Shewbridge, and Roseanna Long for their technical contribution and assistance, Dr. Scott Gahr for his guidance during RNA extraction and cDNA synthesis (supplement A), Dr. J. M. (Hans) Dijkstra for suggestions and for sharing findings from his own research, and Dr. John Hansen for sharing unpublished sequences of the MH-IA and MH-IB genomic regions in rainbow trout.

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  1. Nathan A. Johnson

    Present address: Department of Fisheries and Aquatic Sciences, University of Florida, Gainesville, FL, 32605, USA

Authors and Affiliations

  1. National Center for Cool and Cold Water Aquaculture USDA-ARS, 11861 Leetown Road, Kearneysville, WV, 25430, USA

    Nathan A. Johnson, Roger L. Vallejo, Jeffrey T. Silverstein, Timothy J. Welch, Gregory D. Wiens & Yniv Palti

  2. Department of Fisheries and Wildlife Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061–0321, USA

    Nathan A. Johnson & Eric M. Hallerman

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  1. Nathan A. Johnson
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Correspondence to Yniv Palti.

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Johnson, N.A., Vallejo, R.L., Silverstein, J.T. et al. Suggestive Association of Major Histocompatibility IB Genetic Markers with Resistance to Bacterial Cold Water Disease in Rainbow Trout (Oncorhynchus mykiss). Mar Biotechnol 10, 429–437 (2008). https://doi.org/10.1007/s10126-007-9080-7

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  • DOI: https://doi.org/10.1007/s10126-007-9080-7

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