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Gene expression associated with compatible viral diseases in grapevine cultivars

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Abstract

Viral diseases affect grapevine cultures without inducing any resistance response. Thus, these plants develop systemic diseases and are chronically infected. Molecular events associated with viral compatible infections responsible for disease establishment and symptoms development are poorly understood. In this study, we surveyed viral infection in grapevines at a transcriptional level. Gene expression in the Vitis vinifera red wine cultivars Carménère and Cabernet-Sauvignon naturally infected with GLRaV-3 were evaluated using a genome-wide expression profiling with the Vitis vinifera GeneChip® from Affymetrix. We describe numerous genes that are induced or repressed in viral infected grapevines leaves. Changes in gene expression involved a wide spectrum of biological functions, including processes of translation and protein targeting, metabolism, transport, and cell defense. Considering cellular localization, the membrane and endomembrane systems appeared with the highest number of induced genes, while chloroplastic genes were mostly repressed. As most induced genes associated with the membranous system are involved in transport, the possible effect of virus in this process is discussed. Responses of both cultivars are analyzed and the results are compared with published data from other species. This is the first study of global gene profiling in grapevine in response to viral infections using DNA microarray.

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Acknowledgements

This work was supported by Chilean Genome Initiative FONDEF G02S1001 and INNOVA Bio-Bio Grant AL215L1.

Dr. Cramer acknowledges support from the National Science Foundation (NSF) Plant Genome (DBI-0217653) and the Nevada Agricultural Experiment Station, Publication no. 03066922. The Nevada Genomic Center acknowledges support from the NIH IdeA Network of Biomedical Research Excellence (INBRE, RR-03-008), NIH-NCRR Biomedical Research Infrastructure Network (P20 RR016464) and NSF EPSCoR (EPS-0132556) Integrated Approaches to Abiotic Stress Cluster.

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Authors and Affiliations

  1. Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Alameda 340, Santiago, Chile

    C. Espinoza, A. Vega, C. Medina & P. Arce-Johnson

  2. Department of Genetics and Genomics, Boston University School of Medicine, Boston, MA, 02118, USA

    K. Schlauch

  3. Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557-0014, USA

    G. Cramer

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  1. C. Espinoza
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  2. A. Vega
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  3. C. Medina
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  4. K. Schlauch
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  5. G. Cramer
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  6. P. Arce-Johnson
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Correspondence to P. Arce-Johnson.

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Table S1

Change in gene expression level in Vitis vinifera cv. Carménère during infection with GLRaV-3. After data processing and analysis (for details, see Materials and methods) a group of genes with change in their absolute expression level value more than twofold was obtained. This list includes both up- and down-regulated genes (PDF 83 kb)

Table S2

Change in gene expression level in Vitis vinifera cv. Cabernet-Sauvignon during infection with GLRaV-3. After data processing and analysis (for details, see Materials and methods) a group of genes with change in their absolute expression level value more than twofold was obtained. This list includes both up- and down-regulated genes (PDF 116 kb)

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Espinoza, C., Vega, A., Medina, C. et al. Gene expression associated with compatible viral diseases in grapevine cultivars. Funct Integr Genomics 7, 95–110 (2007). https://doi.org/10.1007/s10142-006-0031-6

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  • DOI: https://doi.org/10.1007/s10142-006-0031-6

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