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Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney

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Abstract

Background

Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.

Methods

Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 105 cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.

Results

The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.

Conclusion

Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.

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Acknowledgments

The work was financially supported in part by grants-in-aids from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Division of Blood Purification, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, 920-8641, Japan

    Kengo Furuichi

  2. Department of Disease Control and Homeostasis, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan

    Hidemi Shintani & Shuichi Kaneko

  3. Department of Laboratory Medicine, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan

    Yoshio Sakai & Takashi Wada

  4. Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan

    Takahiro Ochiya

  5. Department of Molecular Preventive Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

    Kouji Matsushima

Authors
  1. Kengo Furuichi
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  2. Hidemi Shintani
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  3. Yoshio Sakai
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  4. Takahiro Ochiya
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  5. Kouji Matsushima
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  6. Shuichi Kaneko
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  7. Takashi Wada
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Corresponding author

Correspondence to Kengo Furuichi.

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Furuichi, K., Shintani, H., Sakai, Y. et al. Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney. Clin Exp Nephrol 16, 679–689 (2012). https://doi.org/10.1007/s10157-012-0614-6

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  • DOI: https://doi.org/10.1007/s10157-012-0614-6

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